Methods and containment system for storing, reconstituting, disp

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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222 94, 435284, 435296, 435810, C12M 124

Patent

active

053626427

ABSTRACT:
A cell culture media containment system is provided having a mixing bag substantially enclosed within a storage bag. Powdered cell culture media and other constituents are introduced into the mixing bag and are mixed therein. Thereafter, the reconstituted media is conveyed from the mixing bag into the storage bag undergoing sterilization during conveyance therebetween. The reconstituted cell culture media may then be stored in a sterilized condition in the storage bag or maybe dispensed into a cell growth container. In another embodiment of the present invention a plurality of storage bags or mixing bags may be utilized so that reconstituted cell culture media may be both mixed and dispensed while protein-laden cell culture media may be simultaneously harvested back into another of the plurality of storage bags.

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