Methods and compounds for polynucleotide synthesis

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S025330

Reexamination Certificate

active

07572907

ABSTRACT:
Methods of forming polynucleotides are disclosed.

REFERENCES:
Sierzchala et al. “Solid-Phase Oligodeoxynucleotide Synthesis: A two Step Cycle Using Peroxy Anion Deprotection”, Journal of American Chemical Society, 125, 13427-13441, 2003.
Europeán Search Report dated Jul. 27, 2006, 5 pages.
Sierzchala Agnieszka B. et al., Solid-phase oligodeoxynucleotide synthesis: A two-step cycle using peroxy anion deprotection: Journal of the American Chemical Society, vol. 125, No. 44, Nov. 5, 2003 pp. 13427-13441.
Beaucage S. L., et al. “Advances in the Synthesis of Oligonucleotides by the Phosphoramidite Approach” Tetrahedron, Elsevier Science Publishers, Amsterdam, NL, vol. 48, No. 12, 1992, pp. 2223-2311.
Seio, K, et al., “Syntesis of pentathymidylate using a 4-monomethoxytritylthio (MMTrS) group as a 5′-hydroxyl protecting group: toward oligonucleotide synthesis without acid treatment” Tetrahedron Letters, Elsevier, Amsterdam, NL, vol. 42, No. 49, Dec. 3, 2001, pp. 8657-8660.

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