Methods and compositions useful in the recognition, binding and

Details Methods and compositions useful in the recognition, binding and Methods and compositions useful in the recognition, binding and

1992-05-11

1995-08-22

Ziska, Suzanne E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

530350, C07K 1400

Patent

active

054441498

ABSTRACT:
A peptide, Hel-N1 (SEQ ID NO: 2), which can bind to a 3'-untranslated mRNA sequence (which encompasses the "instability sequence") that is uniquely present in the messenger RNAs that encode oncoproteins and lymphokines, and mediates the specific destruction of the messenger RNAs, is described. Full-length Hel-N1 is capable of suppressing cell growth and causing cellular differentiation. Hel-N1 (SEQ ID NO: 2) possess three RNA recognition motifs. One of these forms an RNA-binding domain which, when transfected alone into cells, causes them to undergo rapid growth.

REFERENCES:
Szuho et al. Cell 67:325, 1991.
Levori et al. TIBS 16:214, 1991 (Jun.).
Cobianchi et al. JBC 261(8):3536, 1986.
Rohinow et al. Science 242:1570, 1988.
Patent Cooperation Treaty (Search Report).
PCT Written Option.
The Journal of Biological Chemistry, vol. 261, No. 8 Mar. 15, pp. 3536-3543, (1986).
Annu. Rev. Genet., vol. 24, pp. 519-541, 1990, J. A. Atwater, et al., "Regulated mRNA Stability".
Science, vol. 240, pp. 889-895, May 13, 1988, R. M. Evans, "The Steroid and Thyroid Hormone Receptor Superfamily".
Biochemistry, vol. 19, pp. 4674-4682, 1980, R. L. Karpel, et al., "Physical Studies of the Interaction of a Calf Thymus Helix-Destabilizing Protein with Nucleic Acids".
Cell, vol. 34, pp. 865-879, Oct. 1983, K. Zinn, et al., "Identification of Two Distinct Regulatory Regions Adjacent to the Human Beta-Interferon Gene".
Cell, vol. 45, pp. 827-835, Jun. 20, 1986, A. B. Sachs, et al., "A Single Gene from Yeast for both Nuclear and Cytoplasmic Polyadenylate-Binding Proteins: Domain Strucutre and Expression".
Cell, vol. 46, pp. 659-667, Aug. 29, 1986, G. Shaw, et al., "A Conserved Au Sequence from the 3' Untranslated Region of GM-CSF mRNA Mediates Selective mRNA Degradation".
Cell, vol. 51, pp. 211-220, Oct. 23, 1987, C. C. Query, et al., "A Human Autoimmune Protein Associated with U1 RNA Contains A Region of Homology that is Cross-Reactive with Retrovial p30.sup.gag Antigen".
Cell, vol. 52, pp. 1-3, Jan. 15, 1988, R. M. Evans, et al., "Zinc Fingers: Gilt by Association".
Cell, vol. 52, pp. 221-228, Jan. 29, 1988, J. Wilusz, et al., "A 64 kd Nuclear Protein Binds to RNA Segments that Include the AAUAAA Polydenylation Motif".
Cell, vol. 55, pp. 537-540, Nov. 18, 1988, M. Levine, et al., "Homeobox Proteins as Sequence-Specific Transcription Factors".
Cell, vol. 55, pp. 1025-1035, Dec. 23, 1988, H. Amrein, et al., "The Sex-Determining Gene tra-2 of Drosophila Encodes A Putative RNA Binding Protein".
Cell, vol. 55, pp. 1037-1046, Dec. 23, 1988, L. R. Bell, et al., "Sex-Lethal, A Drosophila Sex Determination Switch Gene, Exhibits Sex-Specific RNA Splicing and Sequence Similarity to RNA Binding Proteins".
Cell, vol. 56, pp. 1011-1018, Mar. 24, 1989, T. J. Goralski, et al., "The Sex Determination Locus Transformer-2 of Drosophila Encodes A Polypeptide with Similarity to RNA Binding Proteins".
Cell, vol. 57, pp. 89-101, Apr. 7, 1989, C. C. Query, et al., "A Common RNA Recognition Motif Identified Within A Defined U1 RNA Binding Domain of the 70K U1 snRNP Protein".
Cell, vol. 58, pp. 231-232, Jul. 28, 1989, M. Rebagliati, "An RNA Recognition Motif in the Bicoid Protein".
Cell, vol. 58, pp. 857-867, Sep. 8, 1989, A. B. Sachs, et al., "The Poly(A) Binding Protein is Required for Poly(A) Shortening and 60s Ribosomal Subunit-Dependent Translation Initiation".
Cell, vol. 67, pp. 325-333, Oct. 18, 1991, A. Szabo, et al., "HuD, A Paraneoplastic Encephalomyelitis Antigen, Contains RNA-Binding Domains and is Homologous to Elav and Sex-Lethal".
Current Option in Cell Biology, vol. 1, pp. 1148-1153, 1989, D. W. Cleveland, "Gene Regulation Through Messenger RNA Stability".
The EMBO Journal, vol. 5, No. 12, pp. 3209-3217, 1986, H. Theissen, et al., "Cloning of the Human cDNA for the U1 RNA-Associated OK Protein".
The EMBO Journal, vol. 6, No. 12, pp. 3841-3848, 1987, P. T. G. Sillekens, et al., "cDNA Cloning of the Human U1 snRNA-Associated A Protein: Extensive Homology Between U1 and U2 snRNP-Specific Proteins".
The EMBO Journal, vol. 7, No. 11, pp. 3519-3529, 1988, M. S. Swanson, et al., "RNA Binding Specificity of the hnRNP Proteins: A Subset Bind to the 3' End of Introns".
The EMBO Journal, vol. 7, No. 13, pp. 4311-4321, 1988, M. Etzerodt, et al., "Structure and Expression of a Xenopus Gene Encoding an snRNP Protein" (U1 70K).
The EMBO Journal, vol. 8, No. 13, pp. 4163-4170, 1989, D. Scherly, et al., "Identification of the RNA Binding Segment of Human U1 A Protein and Definition of its Binding Site on U1 snRNA".
The EMBO Journal, vol. 9, No. 11, pp. 3533-3538, 1990, R. T. Fremeau, et al., "In Situ Analysis of Myelin Basic Protein Gene Expression in Myelin-Deficient Oligodendrocytes: Antisense hnRNA and Readthrough Transcription".
Eur. J. Biochem., vol. 179, pp. 541-548, 1989, M. Sapp, et al., "Characterization of a 48-kDa Nucleic-Acid Binding Fragment of Nucleolin".
FEBS Letters, vol. 257, No. 2, pp. 373-376, Nov., 1989, A. G. Ghetti, et al., "Secondary Structure Prediction for RNA Binding Domain in RNP Proteins Identifies Beta Alpha Beta as the Main Structural Motif".
Genes & Development, vol. 3, pp. 324-333, 1989, M. Caizergues-Ferrer, et al., "Nucleolin from Xenopus Laevis: cDNA Cloning and Expression During Development".
Journal Of Autoimmunity, vol. 2, pp. 329-334, 1989, J. D. Keene, "Molecular Structure of the La and Ro Autoantigens and their Use In Autoimmune Diagnostics".
The Journal of Biological Chemistry, vol. 259, No. 9, pp. 5907-5914, May 10, 1984, I. Pettersson, et al., "The Structure of Mammalian Small Nuclear Ribonucleoproteins".
The Journal of Biological Chemistry, vol. 261, No. 8, pp. 3536-3543, Mar. 15, 1986, F. Cobianchi, et al., "Structure of Rodent Helix-Destabilizing Protein Revealed by cDNA Cloning".
The Journal of Biological Chemistry, vol. 262, No. 23, pp. 10922-10925, Aug. 15, 1987, B. Bugler, et al., "RNA Binding Fragments from Nucleolin Contain the Ribonucleoprotein Consensus Sequence".
The Journal of Biological Chemistry, vol. 262, No. 35, pp. 17126-17137, Dec. 15, 1987, A. Kumar, et al., "Purification and RNA Binding Properties of a C-Type hnRNP Protein from HeLA Cells".
The Journal of Biological Chemistry, vol. 263, No. 2, pp. 1063-1071, Jan. 15, 1988, F. Cobianchi, et al., "Mammalian Heterogeneous Nuclear Ribonucleoprotein Complex Protein A1".
The Journal of Biological Chemistry, vol. 263, No. 7, pp. 3307-3313, Mar. 5, 1988, B. M. Merrill, et al., "Phenylalanines that are Considered Among Several RNA-Binding Proteins Form Part of a Nucleic Acid-Binding Pocket in the A1 Heterogeneous Nuclear Ribonucleoprotein".
The Journal of Biological Chemistry, vol. 263, No. 26, pp. 12824-12827, Sep. 15, 1988, J. H. Chang, et al., "cDNA and Deduced Primary Structure of Rat Protein B23, A Nucleolar Protein Containing Highly Conserved Sequences".
The Journal of Biological Chemistry, vol. 263, No. 34, pp. 18043-18051, Dec. 5, 1988, J. C. Chambers, et al., "Genomic Structure and Amino Acid Sequence Domains of the Human La Autoantigen".
Journal of Molecular Biology, vol. 200, pp. 627-638, 1988, H. M. Bourbon, et al., "Structure of the Mouse Nucleolin Gene, The Complete Sequence Reveals that Each RNA Binding . . .".
Journal of Molecular Biology, vol. 207, pp. 491-503, 1989, G. Biamonti, et al., "Isolation of an Active Gene Encoding Human hnRNP Protein A1".
Molecular and Cellular Biology, vol. 5, No. 8, pp. 1993-1996, Aug., 1985, A. B. Sachs, et al., "Nuclear Polydenylate-Binding Protein".
Molecular and Cellular Biology, vol. 6, No. 8, pp. 2932-2943, Aug., 1986, S. A. Adam, et al., "mRNA Polyadenylate-Binding Protein: Gene Isolation and Sequencing and Identification of a Ribonucleoprotein Consensus Sequence".
Molecular and Cellular Bilogy, vol. 7, No. 5, pp. 1731-1739, May, 1987, M. S. Swanson, et al., "Primary Structure of Human Nuclear Ribonucleoprotein Particle C . . . ".
Molecular and Cellular Biology, vol. 7, No. 8, pp. 2947-2955, Aug., 1987, A. Y. S. Jong, et al., "Saccharomyces Cerevisiae SSB1 Protein and its Relationship to Nucleolar RNA-Binding Proteins".
Molecular and Cellular Biology, vol. 7, No. 9, pp.

Affiliated with

Also associated with

Rating

Methods and compositions useful in the recognition, binding and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions useful in the recognition, binding and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions useful in the recognition, binding and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2142598