Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-09-19
1998-06-02
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4353201, 435325, 536 231, 536 237, 530350, 4241841, C12P 2106, C12N 1500, C07H 2102, C07H 2104
Patent
active
057598134
ABSTRACT:
The present disclosure relates to selected antigenic proteins obtained from the outer membranes of Moraxella catarrhalis, that are found to have a variety of useful properties. These proteins, termed OMPs ("Outer Membrane Proteins"), are characterized as having molecular weights of about 30 kD, 80 kD and between about 200 and 700 kD, respectively. Studies set forth herein demonstrated that monoclonal antibodies directed against these proteins confer a protective effect against infection by Moraxella catarrhalis organisms in animal models, demonstrating the potential usefulness of such antibodies in conferring passive immunity as well as the potential usefulness of these OMPs, or variants thereof, in the preparation of vaccines. Also disclosed are DNA segments encoding these OMPs, methods for preparing the antigens or variants, through the application of recombinant DNA techniques, as well as diagnostic methods and embodiments.
REFERENCES:
Fundamental Immunology, edited by William Paul, Chapter 8, "Immunogenicity and Antigen Structure", Berzofsky et al, 1993, third edition, pp. 242-243, 1993.
Burgess et al., "Possible Dissociation of the Heparin-Binding and Mitogenic Activities of Heparin-Binding(Acidic Fibroblast) Growth Factor-1 from its Receptor-Binding Activities by site-Directed Mutagenesis of a Single Lysine Residue", The Journal of Cell Biology, vol. 111, Nov. 1990, pp. 2129-2138.
Lazar et al., "Transforming Growth Factor a: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities". Molecular and Cellular Biology, vol. 8, No. 3, Mar. 1988, pp. 1247-1252.
Bowie et al. "Diciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions", Science, vol. 247, 1990, pp. 1306-1310.
Eliasson, "A Protein Antigen Characteristic of Branhamella catarrhalis," Acta path. Microbiol. Scand. Scet. B., 88:281-286, 1980.
Shenep et al., "Further Studies of the Role of Noncapsular Antibody in Protection Against Experimental Haemophilus influenzae Type b Bacteremia," Infection and Immunity, 42(1):257-263, 1983.
Chapman et al., "Development of Bactericidal Antibody During Branhamella catarrhalis Infection," J. of Infect. Dis., 151(5):878-882, 1985.
Yogev & Hansen, "Dissociation of Virulence and Protection from Infection by Mutant Analysis in Haemophilus influenzae Type b," Infection and Immunity, 55(8):1944-1947, 1987.
Black & Wilson, "Immunoglobulin G (IgG) Serological Response to Branhamella catarrhalis in Patients with Acute Bronchopulmonary Infections," J. Clin. Pathol., 41:329-333, 1988.
Bartos & Murphy, "Comparison of the Outer Membrane Proteins of 50 Strains of Branhamella catarrhalis," J. Infect. Dis., 158(4):761-765, 1988.
Consensus, Paediatr. Infect. Dis. J., 8(1):S94-S97, 1989.
Murphy & Loeb, "Isolation of the outer membrane of Branhamella catarrhalis," Mocrobial Pathogenesis, 6:159-174, 1989.
Murphy, "The surface of Branhamella catarrhalis: a systematic approach to the surface antigens of an emerging pathogen," Paeditr. Infect. Dis. J., 8(1):S75-S77, 1989.
Doyle, "Animal models of otitis media: other pathogens," The Pediatric Infectious Disease Journal, 8(1):S45-S47. 1989.
Murphy & Bartos, "Surface-Exposed and Antigenically Conserved Determinants of Outer Membrane Proteins of Branhamella catarrhalis," Infect. Immun., 57(10):2938-2941, 1989.
Beaulieu & Roy, "Construction of a Species-Specific DNA Probe for Branhamella catarrhalis,"89th Annual Meting of the American Society for Microbiology, p. 124, Abstract #D-249, 1989.
Hanson et al., "Expression of the Heat-Modifiable Major Outer Membrane Protein of Haemophilus influenzae Type b is Unrelated to Virulence," Infection and Immunity, 57(6):1639-1646. Jun. 1989.
Murphy, "Studies of the Outer Membrane Proteins of Branhamella catarrhalis,"Am. J. Md., 88(5A):41s-45s, 1990.
Goldblatt et al., "Branhamella catarrhalis: Antigenic Determinants and the Development of the IgG Subclass Response in Childhood," J. Infect. Dis., 162:1128-1135, 1990.
Bhushan & Murphy, "Molecular Characterization of Outer Membrane Protein E. of Branhamella catarrhalis: a Potential Vaccine Candidate," 91st General Meeting of th American Society for Microbiology, p. 57, Abstract #B-191, 1991.
Helminen et al., "Molecular Cloning of a Gene Encoding a Surface-Exposed Outer Membrane Protein of Moraxella Catarrahalis," Abstract of the 1991 ICAA, 31, p. 145 Abstract No. 278, 1991.
International Search Report, mailed 27 Nov., 1992.
Klingman & Murphy, "Identification and purification of the lipooligosaccharide-associated high molecular weight outer membrane protein (HMW-OMP) of Branhamella catarrhalis," Abstract of the 92nd General Meeting of the American Society for Microbilogy, p. 90, Abstract #B-388, 1992.
Srikumar et al., "Monoclonal antibodies specific to porin of Haemophilus influenzae type b: localization of their cognate epitopes and tests of their biological activities," Molecular Microbiology, 6(5):665-676, 1992.
Helminen et al., "A major outer membrane protein of Moraxella catarrhalis is a target for antibodies that enhance pulmonary clearance of the pathogen in an animal model," Infection and Immunity, 61(5):2003-2010, 1991.
Hansen Eric J.
Helminen Merja
Maciver Isobel
Board of Regents , The University of Texas System
Hutzell Paula K.
Navarro Mark
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