Methods and compositions for use in high fidelity polymerase cha

Details Methods and compositions for use in high fidelity polymerase cha Methods and compositions for use in high fidelity polymerase cha

1997-10-30

1999-11-02

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435188, 435194, 536 231, 536 2433, C12P 1934, C12Q 168, C07H 2100, C07H 2102

Patent

active

059768428

ABSTRACT:
Methods and compositions are provided for performing high fidelity polymerase chain reactions. In the subject methods, primer extension products are produced with a low error frequency rate through the use of at least one of unequal concentrations of dNTPs or a melting point reducing agent. Also provided are kits and reagent mixtures for use in the subject methods. The subject invention finds use in a variety of applications, particularly in applications where high fidelity PCR is desired.

REFERENCES:
patent: 5436149 (1995-07-01), Barnes
patent: 5491086 (1996-02-01), Gelfand et al.
patent: 5512462 (1996-04-01), Cheng
Barnes et al Proc. Natl. Acad. Sci. USA vol. 91, pp. 2216-2220, 1994.
Eckert et al Nucleic Acids Research vol. 18. No. 13 pp. 3739-3743, 1990.
Vartanian et al Nucleic Acids Research vol. 24, No. 14 pp. 2627-2631, 1996.
Varadaraj et al Gene vol. 140 pp. 1-5, 1994.
Gelfand PCR Technology Principles and Aplications for DNA Amplification Stockton Press NY pp. 17-22, 1989.
Cheng et al Proc. Natl. Acad. Sci. USA vol. 91, pp. 5695-5699, 1994.
Taylor, Graham et al., "The Polymerase Chain Reaction: New Variations On An Old Theme," Current Opinion In Biotechnology (1995) vol. 6:24-29.
Varadaraj, Kulandaiappan et al., "Denaturants or Cosolvents Improve the Specificity of PCR Amplification of a G+C-rich DNA Using Genetically Engineered DNA Polymerases," Gene (1994) vol. 140:1-5.
Vartanian, Jean-Pierre et al., "Hypermutagenic PCR Involoving All Four Transitions and a Sizeable Proportion of Transversions," Nucleic Acids Research (1996) vol. 24, (14):2627-2631.
Fromant, Michel et al., "Direct Random Mutagenesis of Gene-Sized DNA Fragments Using Polymerase Chain reaction," Analytical Biochemistry (1995) vol. 224:347-353.
Braithwaite, Dan et al., "Compilation, Alignment, and Phylogenetic Relationships of DNA Polymerases," Nucleic Acids Research (1993) vol. 21, (4):787-802.
Barnes, Wayne M., "PCR Amplificaton of up to 35-kb DNA With High Fidelity and High yield From .lambda. Bacteriophage templates," Proc. Natl. Acad. Sci. USA (1994) vol. 91:2216-2220.
Eckert, Kristin et al., "DNA Polymerase Fidelity and the Polymerase Chain Reaction," Cold Spring Harbor Laboratory Press (1991) pp. 17-24, PCR Methods and Applications.
Eckert, Kristin et al., "High Fidelity DNA Synthesis by the Thermus aquatics DNA Polymerase," Nucleic Acids Research (1990) vol. 18, (13):3739-3743.
Ling, Lucy et al., "Optimization of the polymerase Chain Reaction with Regard to Fidelity: Modified T7, Taq, and Vent DNA Polymerases," Cold Spring Harbor Laboratory Press (1991) pp. 63-69, PCR Methods and Applications.

Affiliated with

Also associated with

Rating

Methods and compositions for use in high fidelity polymerase cha does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for use in high fidelity polymerase cha, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for use in high fidelity polymerase cha will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2133472