Methods and compositions for use in analyte detection using...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S024310, C536S024100

Reexamination Certificate

active

07914987

ABSTRACT:
Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods.

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Gullberg et al., “Cytokine detection by antibody-based proximity ligation”, PNAS, Jun. 1, 2004, vol. 101, No. 22, pp. 8420-8424.

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