Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-11-05
1999-07-27
Degen, Nancy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 435 701, 435183, 4353201, 536 231, C12N 1552, C12N 1564, C12P 2102, C07H 2104
Patent
active
059289147
ABSTRACT:
Methods and compositions for transforming cells, resulting in efficient and stable site-specific integration of transgenes, are disclosed. Transformation is achieved by introducing into a cell an acceptor vector, preferably a retroviral vector, which integrates into the genome of the cell. The acceptor vector comprises two incompatible lox sequences, L1 and L2. A donor vector is then introduced into the cell comprising a transgene flanked by the same L1 and L2 sequences. Stable gene transfer is initiated by contacting the lox L1 and L2 sequences with Cre recombinase.
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Bouhassira Eric
Leboulch Philippe
Takekoshi Ken Julian
Westerman Karen
Albert Einstein College of Medicine of Yeshiva University (a Div
Degen Nancy
Massachusetts Institute of Technology
Remillard, Esq. Jane E.
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