Methods and compositions for transferring DNA sequence informati

Details Methods and compositions for transferring DNA sequence informati Methods and compositions for transferring DNA sequence informati

1997-08-12

1999-11-23

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 9141, 435455, 435463, 435465, 435325, 435354, 435366, 536 231, 536 241, 536 242, 536 2433, C12P 1934, C12N 1585, C12N 1564, C07H 2104

Patent

active

059898720

ABSTRACT:
Methods and compositions are provided for transferring DNA sequence information from a first vector to a second vector. In the subject methods, a first vector comprising a region of DNA having a sequence of interest is contacted with a set of three pairs of oligonucleotide primers under conditions sufficient to produce three different PCR products, where each product corresponds to a different reading frame. The oligonucleotide primers comprise a first region of sequence identity with the first vector and a second region permissive of site specific recombination with the second vector. The resultant PCR products are combined with the second vector under conditions sufficient for site specific recombination to occur. Also provided are kits for use in performing the subject methods.

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