Methods and compositions for the treatment and diagnosis of ship

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

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4242551, 424823, 435 691, 435 693, 435 711, 435 712, 530350, 5303879, 5303884, 5303895, 536 237, A61K 3900, A61K 3902, C12P 2106, C07K 300

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active

053364910

ABSTRACT:
Novel compositions are disclosed for use in the treatment or diagnosis of bovine pasteurellosis, commonly referred to as Shipping Fever. Cell-free Pasteurella haemolytica supernatants are employed to provide individual antigen compositions, identified through reaction with sera from naturally-infected or convalescent cattle. In particular, at least seven individual P. haemolytica antigen groups were recognized in cell-free culture supernatants. Purified P. haemolytica supernatant, formulated in a suitable pharmaceutical vaccine composition is shown to elicit a specific immune response, in both cows and rabbits, directed against the individual immunoreactive P. haemolytica polypeptides identified. Also disclosed are novel recombinant cells, plasmids and bacteriophage which include transcriptionally active P. haemolytica antigen genes. Recombinant clones are similarly selected to be reactive with naturally-infected antisera. Examples, and further disclosure, are also provided which demonstrate the utility of a presently disclosed antibody and antigen compositions in immuno-detection of both antigens and antibodies in various biological samples.

REFERENCES:
patent: 5055400 (1991-10-01), Lo et al.
patent: 5165924 (1992-11-01), Shewen et al.
Shewen et al Am J. Vet Res 46;1212-1214, 1985.
Himmel Am J. Vet Res 43: 764-767, 1982.
Baluyat et al Am J. Vet Res 42:1920-1926, 1981.
Lo et al Inf & Imm Dec. 3 1985 pp. 667-671 Cloning & Expression of the leukotoxin Gene of Pasteurella haemolytice Al in E. col K-12.
Shewen, Patricia E., "Immunity to Pasteurella haemolytice Serotype 1," North American Symposium on Bovine Respiratory Disease, Amarillo, Tex., Sep. 1983, pp. 480-481. Abstract only.

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