Methods and compositions for the simultaneous detection of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S006120, C435S007100, C435S007200, C435S007920, C435S038000, C435S971000, C435S973000, C436S063000, C436S518000, C436S523000, C436S524000, C436S528000, C436S537000, C436S010000, C436S056000, C436S164000, C436S172000

Reexamination Certificate

active

07659085

ABSTRACT:
Methods and compositions comprising immunoassays for the detection of antigens and antibodies in a sample are described. In particular, the present invention provides assays that are useful for the rapid and simultaneous detection of multiple different antigens and antibodies. In preferred embodiments, the assays include fluorescent labels of multiple wavelengths or intensities, which are used to label the antigens and antibodies directly and to label beads coated with molecules specific for the antigen or antibody. The detection of a fluorescence shift indicates the presence or identity of the antigen or antibody in the sample.

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patent: 6524793 (2003-02-01), Chandler et al.
patent: 2003/0008410 (2003-01-01), Hechinger
patent: WO 99/36564 (1999-07-01), None
patent: WO 99/64867 (1999-12-01), None
Martinez et al. A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies after Vaccination with th 23-valent Pneumococcai Polysaccharide Vaccine (Clinical and Diagnostic Laboratory Immunology 6 (4): 581-586 (Jul. 1999)).
Carson et al. Simultaneous quantitation of 15 cytokines using multiplexed flow cytometry assay (Journal of Immunological Methods 227: 41-52 (1999)).
Carson et al., “Simultaneous Quantitation of 15 Cytokines Using a Multiplexed Flow Cytometric Assay”,Journal of Immunological Methods, Apr. 21, 1999, pp. 41-52, © 1999 Elsevier Science B.V.
Kettman et al., “Classificiation and Properties of 64 Multiplexed Microsphere Sets”,Cytometry, Jun. 10, 1998, pp. 234-243, Cytometry 33 © 1998 Wiley-Liss, Inc.
Park et al., “A Latex Bead-Based Row Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)”,Clinical and Diagnostic Laboratory Immunology, May 2000, pp. 486-489, vol. 7, No. 3, © 2000, American Society of Microbiology.
Martinez et al., “A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine”,Clinical and Diagnostic Laboratory Immunology, Jul. 1999, pp. 581-586, vol. 6, No. 4.

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