Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Reexamination Certificate
2006-02-07
2006-02-07
Sisson, Bradley L. (Department: 1634)
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
C435S287200, C536S023100
Reexamination Certificate
active
06995020
ABSTRACT:
This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or ‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format.
REFERENCES:
patent: 6580056 (2003-06-01), Tacha
patent: 2004/0038270 (2004-02-01), Muhlhahn et al.
Engel et al., Journal of Clinical Pathology, Jan. 1997, vol. 50, No. 1, pp. 37-39.
Baschong et al., The Journal of Histochemistry & Cytochemistry, 2001, vol. 49, No. 12, pp. 1565-1571.
International Search Report corresponding to PCT/US04/23207 mailed Sep. 19, 2005.
Capodieci Paola
Edelson Jon
Aureon Laboratories, Inc.
Gervase, Esq. Richard G.
Mintz Levin Cohn Ferris Glovsky and Popeo PC
Sisson Bradley L.
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