Methods and compositions for the expression of biologically acti

Details Methods and compositions for the expression of biologically acti Methods and compositions for the expression of biologically acti

1991-01-10

1993-08-31

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352523, 43525233, 4353201, 435189, 935 6, 935 10, 935 14, 935 44, 935 45, 935 72, 935 73, C12N 1500, C12N 1553, C12N 1567, C12N 1570

Patent

active

052408310

ABSTRACT:
Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes in a biologically active form. Particular embodiments involve the expression of eukaryotic cythochrome P450.sub.17.alpha. -hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Techniques are disclosed for effecting improved bacterial expression which are proposed to be useful in connection with all eukaryotic cytochrome P450 enzymes which one may desire to express in bacterial hosts such as E. coli and others.

REFERENCES:
patent: 4766068 (1988-08-01), Oeda et al.
patent: 5045471 (1981-09-01), Miller
White, P. C., et al., 1984 Proceedings of the National Academy of Sciences USA, 81:1986-1990.
Evans, L. T., et al., 1986, Proceedings of the National Academy of Sciences USA 83:6387-6391.
Navhi; L. O., et al., 1988, Molecular and Cellular Biochemistry 79(1): 63-72 (abstract).
Porter, T. P., et al., 1987, Archives of Biochemistry and Biophysics 244(1):353-367.
Mittal, S., et al., 1988, Archives of Biochemistry and Biophysics, 264(2):383-391.
Koga, H., et al., 1985, Biophysical and Biochemical Research Communications 130(1):412-417.
Porter et al., Proceedings of the VIIIth International Symposium on Microsomes and Drug Oxidations, Karolinska Institutet, Stockholm, Jun. 25-29, 1990, Eds. M. Ingelman-Sundberg, J-.ANG.. Gustafsson, S. Orrenius, Abstract L-35.
Feyereisen et al., PNAS, 86:1465-1469, 1989.
Andersson et al., J. Bio. Chem., 264(14):8222-8229, 1989.
Cullin and Pompon, Gene, 65:203-217, 1988.
Corbin et al., PNAS, 85:8948-8952, 1988.
Hardwick et al., J. Biol. Chem., 262(2):801-810, 1987.
Chua et al., PNAS, 84:7193-7197, 1987.
Zuber et al., J Biol Chem, 261(5):2475-2482, 1986.
Yoshioka et al., J Biol Chem, 261(9):4106-4109, 1986.
Gonzalez et al., J. Bio. Chem., 260(12):7435-7441, 1985.
Morohashi et al., PNAS, 81:4647-4651, 1984.
Yabusaki et al., Nuc Acid Res., 12(6):2929-2938, 1986.
Fujii-Kuriyama et al., PNAS, 79:2793-2797, 1982.
McCarthy and Gualerzi, Trends Genetics, 6(3):78-85, 1990.
Gold and Stormo, Methods in Enzymology, 185:89-93, 1990.
DeBoer and Hui, Methods in Enzymology, 185:103-114, 1990.
Chen and Inouye, Nucl Acid Res, 18(6):1465-, 1990. (Abstract only).
Gold and Stormo, `E. coli and Salmonella typhimerium Cellular and Molecular Biology`, American Society for Microbiology, Washington, D.C., Ed. F. C. Weidhardt, 1987, pp. 1302-1307.
Bachmair et al., Science, 234:179-, 1988. (Abstract only).
Gren, Biochimie, 66:1-29, 1984.
Stormo et al., Nucl Acid Res, 10(9):2971-2975, 1982.
Platt, Ann Rev Biochem, 55:339-372, 1986.
Hawley and McClure, Nucl Acid Res, 11(8):2237-2255, 1983.
Rosenberg and Court, Ann Rev Genet, 13:319-353, 1979.
Kawasaki, PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., 1990, pp. 21-27.
Higuchi et al., Nucl Acid Res, 16(15):7351-7367, 1988. (Abstract only).
Dialog Search References.
Hu & Chung, Mol. Endocrinology, 4(6):893-989, 1990).
Omer et al., J. Bacteriol., 172(6):3335-3345, 1990.
Imai et al., PNAS, 86:7823-7827, 1989.
Wen & Fulco, J. Biol. Chem., 262(14):6676-6682, 1989.
Unger et al., J. Biol. Chem., 26193:1158-1163, 1986.
Tobias et al., Science, 254, 1374-1377, 1991.
Schein, Current Opinion in Biotechnology, 2:746-750, 1991.
Gottesman, Methods in Enzymology, 185:119-129, 1990.
Kopetzki et al., Mol. Gen. Genet., 216:149-155, 1989.
Kulisch & Vilker, Biotechnol. Prog., 7:93-98, 1991.
Ohkawa et al., Ann. N.Y. Acad. Sci., 613:37-43, 1990.
Halpert et al., Biochem. Pharmacol., 37(15):3046-3048, 1988.
Zuber et al., PNAS, 85:699-703, 1988.
Megges et al., in Molecular Mechanisms of Adrenal Steroidogenesis and Aspects of Regulation and Application, K. Ruckpaul & H. Rein, Eds., Akademie -Verlag, Berlin,
The U.S. government may own certain rights in the present invention pursuant to NIH grant GM37942.

Affiliated with

Also associated with

Rating

Methods and compositions for the expression of biologically acti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for the expression of biologically acti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for the expression of biologically acti will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2296855