Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2006-02-21
2006-02-21
Saucier, Sandra E. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S377000
Reexamination Certificate
active
07001746
ABSTRACT:
The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolated from adipose tissue into adipocytes bearing biochemical, genetic, and physiological characteristics similar to that observed in isolated primary adipocytes. The methods of the invention comprise incubating isolated human preadipocytes, plated at least about 25,000 cells/cm2, in a medium containing, glucose, a cyclic AMP inducer such as isobutylmethylxanthine or forskolin, a glucocorticoid or glucocorticoid analogue, insulin or an insulin analogue and a PPARγ agonist or a RXR agonist. The compositions of the invention include media for the differentiation of human preadipocytes, human adipocytes differentiated by the methods of the invention and transfected adipocytes.The present invention also provides methods for determining the ability of a compound to affect the differentiation of human preadipocytes to adipocytes, for determining the ability of a compound to act as a PPARγ antagonist. a glucocorticoid, a glucocoticoid analogue, or an insulin analogue, for transfecting cultured human adipocytes, and as a means to identify novel polypeptides secreted from human adipocytes into the conditioned medium. The methods and compositions have use in the drug discovery of compounds having relevance to the disease states of diabetes, obesity, and cardiovascular disease and in the studies of these diseases.
REFERENCES:
patent: 5041138 (1991-08-01), Vacanti et al.
patent: 5144016 (1992-09-01), Skjak-Braek et al.
patent: 5486359 (1996-01-01), Caplan et al.
patent: 5516532 (1996-05-01), Atala et al.
patent: 5536656 (1996-07-01), Kemp et al.
patent: 5709854 (1998-01-01), Griffith-Cima et al.
patent: 5723331 (1998-03-01), Tubo et al.
patent: 5736372 (1998-04-01), Vacanti et al.
patent: 5736396 (1998-04-01), Bruder et al.
patent: 5741685 (1998-04-01), Vacanti
patent: 5759830 (1998-06-01), Vacanti et al.
patent: 5770193 (1998-06-01), Vacanti et al.
patent: 5770417 (1998-06-01), Vacanti et al.
patent: 5786207 (1998-07-01), Katz et al.
patent: 5804178 (1998-09-01), Vacanti et al.
patent: 5811094 (1998-09-01), Caplan et al.
patent: 5827735 (1998-10-01), Young et al.
patent: 5855610 (1999-01-01), Vacanti et al.
patent: 5863531 (1999-01-01), Naughton et al.
patent: 5879940 (1999-03-01), Torok-Storb et al.
patent: 5902741 (1999-05-01), Purchio et al.
patent: 5908784 (1999-06-01), Johnstone et al.
patent: 5942225 (1999-08-01), Bruder et al.
patent: 5944754 (1999-08-01), Vacanti
patent: 6027744 (2000-02-01), Vacanti et al.
patent: 6030836 (2000-02-01), Thiede et al.
patent: 6071747 (2000-06-01), Strosberg et al.
patent: 6103522 (2000-08-01), Torok-Storb et al.
patent: 6123727 (2000-09-01), Vacanti et al.
patent: 6139574 (2000-10-01), Vacanti et al.
patent: 6143501 (2000-11-01), Sittinger et al.
patent: 6153432 (2000-11-01), Halvorsen et al.
patent: 6200606 (2001-03-01), Peterson et al.
patent: 6242200 (2001-06-01), Wilkison et al.
patent: 6316247 (2001-11-01), Katz et al.
patent: WO 96/02662 (1996-02-01), None
patent: WO 98/20731 (1998-05-01), None
patent: WO 99/28444 (1999-06-01), None
patent: WO 99/43286 (1999-09-01), None
patent: WO 99/61587 (1999-12-01), None
patent: WO 00/44882 (2000-08-01), None
patent: WO 00/53795 (2000-09-01), None
patent: WO 01/21767 (2001-03-01), None
Gibco Catalog p. 688 (1992).
Zilberfarb et al., “Human immortalized brown adipocytes express functional beta 3-adrenoceptor coupled to lipolysis”, J. Cell Science 110: 801-807 (1997).
Schmidt et al., “Adipose Conversion of 3T3-L1 Cells in a Serum-free Culture System Depends on Epidermal Growth Factor, Insulin-like Growth Factor 1, Corticosterons, and Cyclic AMP,” (1990), The Journal of Biological Chemistry, vol. 265:26, pp. 15489-15495.
Wu et al., “Induction of Peroxisome Proliferator-Activated Receptor γ during the Conversion of 3T3 Fibroblasts into Adipocytes Is Mediated by C/BBPβ, C/BBPδ, and Glucocorticoids,” Molecular and Cellular Biology, (1996), vol. 16:8, p. 4128-4136.
Arner, “The β3-Adrenergic Receptor—A Cause and Cure of Obesity?”New England Journal of Medicine, (1995), 333:382-383.
Barham et al., “An Improved Colour Reagent for the Determination Blood Glucose by the Oxidase System,”Analyst, (1972) 97:142-145.
Becker et al., “Use of Recombinant Adenovirus for Metabolic Engineering of Mammalian Cells,”Meth Cell Biol, (1994) 43:161-189.
Digby et al., “Thiazolidinedione Exposure Increases the Expression of Uncoupling Protein 1 in Cultured Human Preadipocytes,”Diabetes, (1998), 47:138-141.
Dulbecco et al., “Plaque Production by the Polyoma Virus,”Virology, (1959) 8:396.
Earle, “Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells,”JNCI, (1943), 4:165-169.
Felgner et al., “Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure,”Proc Natl Acad Sci USA, (1987), 84:7413-7417.
Flier, “The Adipocyte: Storage Depot or Node on the Energy Information Superhighway?”Cell, (1995), 80:15-18.
Green et al., “Sublines of Mouse 3T3 Cells That Accumulate Lipid,”Cell, (1974), 1:113-116.
Ham, “An Improved Nutrient solution for Diploid Chinese Hamster and Human Cell Lines,”Exp Cell Res., (1963) 29:515-526.
Hauner et al., “Promoting Effect of Glucocorticoids on the Differentiation of Human Adipocyte Precursor Cells Cultured in a Chemically Defined Medium,”Journal Clin Invest., (1989) 34:1663-1670.
Heyman et al., “9-Cis Retinoic Acid Is a High Affinity Ligand for the Retinoid X Receptor,”Cell, (1992), 68:397-406.
Hotamisligil et al., “Increased Adipose Tissue Expression of Tumor Necrosis Factor-α in Human Obesity and insulin Resistance,”J. Clin. Invest., (1995), 95:2409-2415.
Lehmann et al., “An Antidiabetic Thiazolidinedione Is a High Affinity Ligand for Peroxisome Proliferator-activated Receptor γ(PPARγ)*,”J. Biological Chemistry, (1995), 270:12953-12956.
Meunier-Durmont et al., “Efficient transfer of regulated genes in adipocytes and hepatoma cells by the combination of liposomes and replication-deficient adenovirus,”Eur Jour Biochem, (1996), 237:660-667.
Morton, “A Survey of Commercially Available Tisue Culture Media,”In Vitro, (1970) 6:89-108.
Nolan et al., “Improvement in Glucose tolerance and Insulin Resistance in Obese Subjects Treated with Troglitazone,”New England Journal of Medicine, (1994) 331:1188-1193.
Pelleymounter et al., “Effects of the obese Gene Product on Body Weight Regulation in ob/ob Mice,”Science, (1995), 269:540-543.
Rodbell et al., “[7] Preparation of Isolated Fat Cells and Fat Cell “Ghosts”; Methods for Assaying Adenylate Cyclase Activity and Levels of Cyclic AMP,”Method in Enzyrology, (1967) 31:104-114.
Rodbell, “Metabolism of Isolated Fat Cells v. Preparationof “Ghosts” and their Properties; Adenyl Cyclase and Other Enzymes,”Journal of Biol Chem., (1967) 242:5744-5750.
Saltiel et al., “Perspectives in Diabetes—Thiazolidinediones in the Treatment of Insulin Resistance and Type II Diabetes,”Diabetes, (1996), 45:1661-1669.
Smith et al., “The Nucleic acid of Polyoma Virus,”Virology, (1960) 12:185-196.
Sreenan et al., “Prevention of hyperglycemia in the Zucker diabetic fatty rat by treatment with metformin or troglitazone,”American Journal Physiol, (1996), 271:E742-E747.
Tontonoz et al., “Stimulation of Adipogenesis in Fibroblasts by PPARγ2, a Lipid-Activated Transcription Factor,”Cell, (1994), 79:1147-1156.
Wigler et al., “Transfer of Purified Herpes Virus Thymidine Kinase Gene to cultured Mouse Cells,”Cell, (1977), 11:223-232.
Zhang et al., “Positional cloning of the mouseobesegene and its human homologue,”Natu
Halvorsen Yuan-Di Chang
Wilkison William O.
Artecel Sciences, Inc.
Morgan & Lewis & Bockius, LLP
Saucier Sandra E.
LandOfFree
Methods and compositions for the differentiation of human... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for the differentiation of human..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for the differentiation of human... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3659238