Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1981-12-03
1983-12-27
Marantz, Sidney
Chemistry: analytical and immunological testing
Biological cellular material tested
435 6, 436174, G01N 3352
Patent
active
044231539
ABSTRACT:
Fluorochrome compositions and methods for the selective detection and determination of double stranded, helical DNA are provided. DNA is quantitated by determining the fluorescence enhancement imparted from the formation of a highly specific DNA-fluorochrome adduct. The fluorochrome composition disclosed includes a divalent metallic cation and a fluorescent antibiotic, antineoplastic agent selected from mithramycin, chromomycin or olivomycin. A compatible surfactant can also be included with the fluorochrome composition to enhance solubilization of cellular matter. Moreover, a compatible membrane penetrating agent such as dimethyl sulfoxide can be included with the composition to accelerate solubilization of the cellular matter. The compositions and methods provided are also useful for the screening or quantitating of agents which interact with DNA to induce displacement of fluorochrome bound to DNA.
REFERENCES:
patent: 4302437 (1981-11-01), Herbert
patent: 4345027 (1982-08-01), Dolbeare
B. T. Hill, Anal. Biochem., 70(2), 635-638 (1976).
Chemical Abstracts, 88:185709w (1978).
Quattrone Alfred J.
Ranney David F.
Board of Regents , The University of Texas System
Marantz Sidney
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