Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Reexamination Certificate
2011-07-05
2011-07-05
Menon, Krishnan S (Department: 1777)
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
C250S458100, C536S024300
Reexamination Certificate
active
07972860
ABSTRACT:
The present invention relates to a novel integrated PCR-free signal amplification polynucleotide detection system which combines a specific receptor, an optical transducer, and an amplification mechanism. This novel detection system is based on different electrostatic interactions and confirmations between a cationic polythiophene (i.e., polymer 1) and single-stranded or double-stranded polynucleotides (such as ss-DNA or ds-DNA), and the efficient energy transfer between the triplex (complexation between the cationic polythiophene and ds-DNA) and neighboring fluorophores attached to ss-DNA or ds-DNA probes. It is to be understood that in the case of ss-DNA, triplex formation occurs via the hybridization of complementary ss-DNA strands, combined with complexation and with the cationic polythiophene. The present detection system allows for the detection of single nucleotide polymorphisms (SNPs) from samples in only a few minutes, without the need for nucleic acid amplification.
REFERENCES:
patent: 5780610 (1998-07-01), Collins
patent: 6197949 (2001-03-01), Teoule et al.
patent: 6589731 (2003-07-01), Chen et al.
patent: 7214489 (2007-05-01), Bazan et al.
patent: 7270956 (2007-09-01), Bazan et al.
patent: 2004/0142206 (2004-07-01), Bazan et al.
patent: 2005/0003386 (2005-01-01), Bazan et al.
patent: WO 98/03499 (1998-01-01), None
patent: WO 02/081735 (2002-10-01), None
Dore et al. “Fluorescent polymeric transducer for the rapid, simple, and specific detection of nucleic acids at the zeptomole level” J. Am. Chem. Soc., 126(13), pp. 4240-4244 (2004).
Nilsson et al. “Chip and solution detection of DNA hybridization using a luminescent zwitterionic polythiophene derivative” Nat. Mater., 2(6), pp. 419-424 (2003).
Drummond et al. “Electrochemical DNA sensors” Nature Biotechnology, 21(10), pp. 1192-1199 (2003).
Kim et al. “Control of conformational and interpolymer effects in conjugated polymers” Nature, 411, pp. 1030-1034 (2001).
Leach et al. “Theoretical investigation of novel nucleic acid bases” J. Am. Chem. Soc., 114(10), pp. 3675-3683 (1992).
Levitsky et al. “Energy migration in poly(phenylene ethynylene): Determination of interpolymer transport in anisotropic Langmuir-Blodgett films” J. Am. Chem. Soc., 121(7), pp. 1466-1472 (1999).
Liu et al. “Homogenous fluorescence-based DNA detection with water-soluble conjugated polymers” Chem. Mater., 16(23), pp. 4467-4476 (2004).
Liu et al. “Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection” Anal. Chem., 76(7), pp. 1824-1831 (2004).
McQuade et al. “Signal amplification of a ‘turn-on’ sensor: Harvesting the light captured by a conjugated polymer” J. Am. Chem. Soc., 122(49), pp. 12389-12390 (2000).
Nam et al. “Bio-bar-code-based DNA detection with PCR-like sensitivity” J. Am. Chem. Soc., 126(19), pp. 5932-5933 (2004).
Phaneuf et al. “Type 1 hereditary tyrosinemia. Evidence for molecular heterogeneity and identification of a causal mutation in a French Canadian patient” J. Clin, Invest. 90, pp. 1185-1192 (1992).
Piccirilli et al. “Enzymatic incorporation of a new base pair into DNA and RNA extends the genetic alphabet” Nature, 343, pp. 33-37 (1990).
Saiki et al. “Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia” Science, 230, pp. 1350-1354 (1985).
St-Louis et al. “Mutations in the fumarylacetoacetate hydrolase gene causing hereditary tyrosinemia type 1: overview” Human Mutation 9, pp. 291-299 (1997).
Switzer et al. “Enzymatic recognition of the base pair between isocytidine and isoguanosine” Biochemistry, 32(39), pp. 10489-10496 (1993).
Tor et al. “Site-specific enzymatic incorporation of an unnatural base, N6-(6-aminohexyl)isoguanosine, into RNA” J. Am. Chem. Soc., 115(11), pp. 4461-4467 (1993).
Mantsch et al. “Structural and enzymatic properties of adenine 1-oxide nucleotides” Biochemistry. 14(26), pp. 5593-5601 (1975).
Beljonne, D., et al., Interchain vs. intrachain energy transfer in acceptor-capped conjugated polymers, Proc. Natl. Acad. Sci. U.S.A. 99, 10982-10987 (2002).
Chen, L., et al., Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer, Proc. Natl. Acad. Sci. U.S.A. 96, 12287-12292 (1999).
Daar, A.S., et al., Top ten biotechnologies for improving health in developing countries, Nat. Genet. 32, 229-232 (2002).
Fodor, S.P.A., et al., Light-directed spatially addressable parallel chemical synthesis, Science 251, 767-773 (1991).
Gaylord, B.S., et al., DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes. Proc. Natl. Acad. Sci. U.S.A. 99, 10954-10957 (2002).
Ho, H.A., et al., Colorimetric and fluorometric detection of nucleic acids using cationic polythiophene derivatives., Angew. Chem. Int. Ed. 41, 1548-1551 (2002).
Leclerc, M., Optical and electrochemical transducers based on functionalized conjugated polymers, Adv. Matter. 11, 1491-1498 (1999).
McQuade, D.T., et. al., Conjugated polymer-based chemical sensors, Chem. Rev. 100, 2537-2574 (2000).
Nguyen, T.-Q., et al., Control of energy transfer in oriented conjugated polymer-mesoporous silica composites, Science 288, 652-656 (2000).
Tyagi, S. & Kramer, F.R., Molecular beacons: probes that fluoresce upon hybridization, Nat. Biotechnology 14, 303-308 (1996).
Ho Hoang-Anh
Leclerc Mario
Fritchman Rebecca
Menon Krishnan S
Milbank Tweed Hadley & McCloy LLP
National Research Council of Canada
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