Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-07-18
2006-07-18
Slobodyansky, Elizabeth (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S098000, C435S099000, C435S100000, C435S200000
Reexamination Certificate
active
07078194
ABSTRACT:
Mutant glycosidase enzymes are formed in which the normal nucleophilic amino acid within the active site has been changed to a nonnucleophilic amino acid. These enzymes cannot hydrolyze disaccharide products, but which can still form them. Using this enzyme, oligosaccharides are synthesized by preparing a mixture of an &agr;glycosyl fluoride and a glycoside acceptor molecule; enzymatically coupling the &agr.glycosyl fluoride to the glycoside acceptor molecule to form a glycosyl glycoside product using the mutant glycosidase enzyme; and recovering the glycosyl glycoside product. Particular enzymes include a mutant form ofAgrobacterium&bgr.Glucosidase in which the normal glutamic acid residue at position 358 is replaced with an alanine residue.
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MacKenzie Lloyd
Wang Qingping
Withers Stephen G.
Slobodyansky Elizabeth
The University of British Columbia
Townsend & Townsend & Crew LLP
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