Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-07-20
1996-07-02
Stone, Jacqueline M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 536 252, 935 78, C12Q 168
Patent
active
055321305
ABSTRACT:
(2'-5') linked 3'-Deoxyoligodeoxynucleotides have been synthesized on a solid support via standard cyanoethyl phosphoramidite chemistry. This simple change in the oligonucleotide bond connectivity leads to unique properties. Thus (2'-5') 3'-deoxyoligodeoxynucleotides hybridize selectively to single stranded RNA but not DNA. The (2'-5') linkages confer greater resistance to exo- and endonyucleolytic degradation compared to (3'-5') -linked oligomers. In addition, (2'-5') linked 3'-deoxyoligodeoxynucleotides support RNase-H activity. The nucleic acid selectivity of (2'-5')oligo-3'0-deoxynucleotides may represent an important design feature to improve the efficacy of antisense oligonucleotides.
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Damha, et al., Nucleic Acids Research, p. 290 Symp. Ser. No. 24, 1991.
Giannaris, et al., Nucleic Acids Research, 4742-49, 21:20, (1993).
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Dougherty et al (1992) J Am Chem Soc 114(15): 6254-5.
Campell Bruce R.
Dyad Pharmaceutical Corporation
Oppenheimer Max Stul
Stone Jacqueline M.
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