Methods and compositions for sensitive and rapid, functional ide

Details Methods and compositions for sensitive and rapid, functional ide Methods and compositions for sensitive and rapid, functional ide

1996-09-26

1999-07-27

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 6, 435455, 435463, 435 691, 435 911, 435230, 435235, 436501, 536 231, 536 243, 536 2431, 536 245, C12Q 102, C12Q 168, C12Q 100, G01N 33566

Patent

active

059288884

ABSTRACT:
The invention provides for a methods and compositions for identifying proteins or compounds that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting a BL (beta-lactamase) expression construct into an eukaryotic genome, usually non-yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.

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