Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2004-05-12
2008-10-14
Strzelecka, Teresa E. (Department: 1637)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C435S006120, C435S018000, C435S975000
Reexamination Certificate
active
07435794
ABSTRACT:
Methods of selectively cleaving DNA containing duplex nucleic acids in a complex nucleic acid mixture, as well as nuclease containing compositions for use therein, are provided. In the subject methods, a nuclease or composition thereof is employed to provide for selective cleavage of DNA containing duplex nucleic acids in a complex nucleic acid mixture. Also provided are novel duplex-stranded specific nucleases and nucleic acids encoding the same, where the subject nucleases are enzymes that, preferentially cleave deoxyribonucleic acid molecules in perfectly matched nucleic acid duplexes as compared to non-perfectly matched nucleic acid duplexes of the same length and/or single stranded nucleic acids. The subject methods and compositions for practicing the same find use in a variety of different applications, including, but not limited to, nucleic acid analyte detection applications, gene expression profiling applications, detection of nucleic acid variants including single nucleotide polymorphisms applications, preparation of subtracted and normalized nucleic acid libraries, etc. Finally, kits for use in practicing the subject methods are provided.
REFERENCES:
GenBank Accession No. CAB55635, “Deoxyribonuclease l [Marsupenaus japonicus]”, Apr. 10, 2000.
Menzorova et al. “Isolation and Characterization of Ca2+Mg2 +-Dependent Endonuclease From Hepatopancreas of the Crab,”Biochemistry(Moscow) (1993) 58:681-691.
Menzorova et al. “Highly Stable Ca2,1+Mg2+-Dependent DNase From Crab Hepatopancreas,”Biochemistry(Moscow) (1994) 59:321-325.
Chou et al. “Shrimp Hepatopancreatic Deoxyribonuclease-Purification and Characterization as Well as Comparison with Bovine Pancreatic Deoxyribonuclease,”Biochemica et Biophysica Acta(1990) 1036:95-100.
Lin et al. “Thermal Inactivation of Shrimp Deoxyribonuclease With and Without Sodium Dodecyl Sulfate,”Biochemica et Biophysica Acta(1994) 1209:209-214.
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Lukyanov Sergey A.
Rebrikov Denis V.
Shagin Dmitriy A.
Bozicevic Field & Francis LLP
Evrogen JSC
Field Bret E.
Strzelecka Teresa E.
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