Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-03-01
1998-11-24
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 6, 4351723, 536 237, 536 241, C12P 2102, C12N 1567, C07H 2104, C12Q 168
Patent
active
058405232
ABSTRACT:
The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
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Simmons Laura C.
Yansura Daniel G.
Degen Nancy
Dreger Walter H.
Genetech, Inc.
Vance Dolly A.
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