Methods and compositions for RNA amplification and detection...

Details Methods and compositions for RNA amplification and detection... Methods and compositions for RNA amplification and detection...

2008-01-29

2008-01-29

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S091510, C435S174000, C536S022100, C530S350000

Reexamination Certificate

active

07323310

ABSTRACT:
Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

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