Methods and compositions for reverse translation

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C435S007100, C536S024100, C930S010000

Reexamination Certificate

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10286796

ABSTRACT:
A process is disclosed by which a polynucleotide is directly synthesized from the peptide or protein that it encodes without the need for sequencing (or sequence analysis) of the peptide or protein. Information contained in the sequence of the peptide or protein is directly coupled, by the process of reverse translation, to the synthesis of the polynucleotide. The usefulness of reverse translation is that it facilitates the amplification of information held in the amino acid sequence (the primary structure) of an unknown protein or peptide. Amplification is useful for, among other things, the identification and/or scientific investigation of the peptide or protein.

REFERENCES:
patent: 4551797 (1985-11-01), Amdahl et al.
patent: 5541342 (1996-07-01), Korhonen et al.
patent: 5786428 (1998-07-01), Arnold et al.
patent: 5843701 (1998-12-01), Gold et al.
patent: 6846638 (2005-01-01), Shipwash
Agrawal, S. (1993) Protocols for Oligonucleotides and Analogs. Humana Press, Totowa, NJ, index only.
Agrawal, S. (1994) Protocols for Oligonucleotide Conjugates. Humana Press, Totowa, NJ.
Ahern, K. (2001) “Variations on PCR for the Amplification of DNA,” Genetic Engineering News 21, 9.
Andersson, L.I. (2000) “Molecular Imprinting For Drug Bioanalysis. A Review On The Application Of Imprinted Polymers To Solid-Phase Extraction And Binding Assay,” J. Chromatogr. B 739, 163-173.
Andersson, L.I. et al. (1990a) “Enantiomeric Resolution Of Amino Acid Derivatives On Molecularly Imprinted Polymers As Monitored By Potentiometric Measurements,” J. Chromatogr. 516, 323-331.
Andersson, L.I. et al. (1990b) “Molecular Recognition In Synthetic Polymers: Preparation Of Chiral Stationary Phases By Molecular Imprinting Of Amino Acid Amides,”J. Chromatogr. 513, 167-179.
Anonymous, Product Catalog and technical literature, Glen Research Corporation, Sterling, VA, 1999.
Avers, C.J. (1976) “Cell Biology,” Van Nostrand, New York, index only.
Bhown, A.S. (1987) “Protein/Peptide Sequence Analysis: Current Methodologies,” Protein/Peptide Sequence Analysis: Current Methodologies, CRC Press, Boca Raton, FL, index only.
Biro, J. (1983) “Information Transfer in Biological Systems,” Med. Hypotheses 12, 31-40.
Blackstock, W.P. & Weir, M.P. (1999) “Proteomics: Quantitative and Physical Mapping of Cellular Proteins,” Trends Biotechnol. 17, 121.
Braco, L. et al. (1990) “Production Of Abiotic Receptors By Molecular Imprinting Of Proteins,” Proc. Natl. Acad. Sci., USA 87, 274-277.
Cook, N.D. (1977) “The Case for Reverse Translation,” J. Theor. Biol. 64, 113.
Craig, R. (1981) “The Theoretical Possibility Of Reverse Translation Of Proteins Into Genes,” J. Theor. Biol. 88, 760.
Dai, S. et al. (1999) “Imprint Coating: A Novel Synthesis of Selective Functionalized Ordered Mesoporous Sorbents,” Angew. Chem. Int. Ed. 38, 1235.
Dhal, P.K. et al. (1995) “Surface Grafting of Functional Polymers to Macroporous Poly(trimethylolpropane) trimethacrylate),” Chem. Mater. 7, 154-162.
Dickert, FL & Thierer S (1996) “Molecularly Imprinted Polymers for Optochemical Sensors,” Adv. Mater. 8, 987.
Dove, A. (1999) “Proteomics: Translating Genomics into Products,” Nature Biotechnol. 17, 233.
Dupont, D.R. et al. (2000) “The Alkylated Thiohydantoin method for C-terminal Sequence Analysis,” EXS 88, 119-131.
Eckstein, F. (1991) Oligonucleotides and Analogues, A Practical Approach. IRL Press, New York.
Findlay, JBC & Geisow, MJ Protein Sequencing. A Practical Approach, Oxford University Press, New York. p. 121-123.
Freitag, R. & Vogt, S. (2000) “Comparison Of Particulate And Continuous-Bed Columns For Protein Displacement Chromatography,” J. Biotechnol. 78, 69-72.
Frenz, J. (1996) “Trace Analysis Peptide Mapping be High-Performance Displacement Chromatography,” Meth. Enzymol. 271, 486-504.
Gevaert, K. et al. (2000) “Techniques for Sample Preparation Including Methods for Concentrating Peptide Samples,” EXS 88, 29-42.
Guillier, F. et al. (2000) “Linkers And Cleavage Strategies In Solid-Phase Organic Synthesis And Combinatorial Chemistry,” Chem. Rev. 100, 2091-2157.
Hames, B.D. Gel Electrophoresis of Proteins: A Practical Approach. Oxford Univ. Press, New York, 1988, p. 189-228.
Hancock, W.S. CRC Handbook of HPLC For the Separation of Amino Acids, Peptides, and Proteins. CRC Press, Boca Raton, Florida, 1984, p. 376-379.
Harlow, E. & Lane, D. (1988) “” Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, index only.
Haugland, R.P. (1996) “,” Handbook of Fluroescent Probes and Research Chemicals. Molecular Probes, Inc., Eugene, OR, index only.
Haupt, K. & Mosbach, K. (2000) “Molecularly Imprinted Polymers And Their Use In Biomimetic Sensors,” Chem. Rev. 100, 2495-2504.
Hellman, U. (2000) “Sample Preparation By SDS/PAGE And In-Gel Digestion,” EXS 88, 43-54.
Hermanson, G.T. (1996) Bioconjugate Techniques, Academic Press, New York, p. 303, 304, 368, 369, 666, 667.
Hermanson, G.T. et al. (1992) Immobilized Affinity Ligand Techniques. Academic Press, New York, index.
Honore, B & Madsen, P. (1997) “The Tetramethylammonium Chloride (TMAC) Method For Screening cDNA Libraries With Highly Degenerate Oligonucleotide Probes Obtained By Reverse Translation Of Amino Acid Sequences,” Methods Mol. Biol. 69, 139.
Humphery-Smith et al. (1997) “Proteome Research: Complementarity And Limitations With Respect To The RNA And DNA Worlds,” Electrophoresis 18, 1217.
Inglis, A.S. (1991) “Chemical Procedures For C-Terminal Sequencing Of Peptides And Proteins,” Anal. Biochem. 195, 183-196.
International Human Genome Sequencing Consortium (2001) “Initial Sequencing and Analysis of the Human Genome,” Nature 409, 860.
Irving, M.B. et al. (2001) “Random-Peptide Libraries And Antigen-Fragment Libraries For Epitope Mapping And The Development Of Vaccines And Diagnostics,” Curr. Opin. Chem. Biol. 5, 314-24.
Kalghatgi, K. et al. (1992) “Rapid Displacement Chromatography Of Melittin On Micropellicular Octadecyl-Silica,” J. Chromatogr. 604, 47-53.
Katz, A. & Davis, M.E. (2000) “Molecular Imprinting of Bulk Microporous Silica,” Nature 403, 286-289.
Kempe, M. & Mosbach, K. (1995) “Separation Of Amino Acids, Peptides, And Proteins On Molecularly Imprinted Stationary Phases,” J. Chromatogr. A 691, 317.
Kirsch, N. et al. (2000) “Enhancement Of Selectivity Of Imprinted Polymers Via Post-Imprinting Modification Of Recognition Sites,” Polymer 41, 5583-5590.
Klein, J.U. et al. (1999) “Template-Mediated Synthesis Of A Polymeric Receptor Specific To Amino Acid Sequences,” Angew. Chem. Int. 38, 2057-60.
Kloor, D. et al. (2000) “Simple And Sensitive Binding Assay For Measurement Of Adenosine Using Reduced S-Adenosylhomocysteine Hydrolase,” Clin. Chem 46, 537-542.
Kundu, A. et al. (1995) “Protected Amino Acids As Novel Low Molecular Weight Displacers In Cation-Exchange Displacement Chromatography,” Biotechnol. Bioengin. 48, 452-460.
Lahm, H.-W. & Langen, H. (2000) “Mass Spectrometry: A Tool For The Identification Of Proteins Separated By Gels,” Electrophoresis 21, 2105-2114.
Lee, S.-W. et al. (1998) “Molecular Imprinting Of Protected Amino Acids In Ultrathin Multilayers Of Tio2Gel,” Chem. Lett. (12) “,” 1193-1194.
Lehninger, A.L. (1975) Biochemistry, 2nd Ed., Worth Publishers, Inc. New York, index only.
Leonhardt, A. & Mosbach, K. (1987) “Enzyme-Mimicking Polymers Exhibiting Specific Substrate Binding And Catalytic Functions,” Reactive Polymers 6, 285.
Lottspeich et al. “Amino Acid Analysis in Microscale,” in Cell Biology: A Laboratory Handbook, 2nd Ed, New York

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