Methods and compositions for reducing false positive signals in

Details Methods and compositions for reducing false positive signals in Methods and compositions for reducing false positive signals in

1992-09-24

1995-11-07

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 9121, 435 913, 435 9132, 435 9151, 435193, 435194, 536 231, 536 253, 935 3, 935 17, 935 19, 935 77, C12Q 168, C12P 1930, C07H 2102

Patent

active

054647444

ABSTRACT:
Disclosed herein is a composition comprising ribonuclease treated RNA or DNA dependent RNA polymerase, and the use of same in amplification methods. The treatment of the RNA or DNA dependent RNA polymerase with ribonuclease reduces or eliminates false positives which result from the presence of an endogenous or contaminating replicatable template species in the Q.beta. replicase enzyme preparation.

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Pritchard et al. (1990) Ann. Biol. Clin., vol. 48, pp. 492-497.
Feix et al. (1967) Proceedings of the Nat'l Acad. of Sciences (USA), vol. 57, No. 5, pp. 1401-1408.
Sumper et al. PNAS 72:162-166, 1975.
Schaffner et al. (1977) J. Mol. Biol., vol. 117, pp, 877-907.
Flanagan et al. (1979) J. of Virology, vol. 29, No. 1, pp. 352-360.
Chifflot et al. (1980) Virology, vol. 100, pp. 91-100.
Lizardi, et al., Trends in Biotech. vol. 9: 53-58 (Feb. 1991).

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