Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Medium – per se – for culture – maintenance – regeneration – etc.
Reexamination Certificate
2007-07-03
2007-07-03
Lankford, L B (Department: 1651)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Medium, per se, for culture, maintenance, regeneration, etc.
C435S410000, C435S420000, C435S430000, C435S430100
Reexamination Certificate
active
10917919
ABSTRACT:
The present disclosure relates to methods and compositions for in vitro cultivation of species ofSwertia, e.g.Swertia chirata(Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation ofSwertia chiratacomprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation ofSwertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal
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Ahuja Ashok
Kaul Brij Lal
Kaul Mahraj Krishen
Koul Sushma
Qazi Ghulam Nabi
Baker & Botts L.L.P.
Lankford L B
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