Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Reexamination Certificate
2005-02-15
2005-02-15
Campell, Bruce R. (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
C435S410000
Reexamination Certificate
active
06855547
ABSTRACT:
The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g.Swertia chirata(Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation ofSwertia chiratacomprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation ofSwertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal applications.
REFERENCES:
Nalawade SM et al., 2003, “Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization”Bot. Bull. Acad. Sin.44:79-98.
Chang C et al., 2000, “A tissue culture protocol for propagation of a rare plant,Lilium speciosum Thunb. var. gloriosoidesBaker”Bot. Bull. Acad. Sin. 41:139-142.
Keil, M et al., 2000, “Production of amarogentin in root cultures ofSwertia chirata” Plant Med. 66:452-457.
Kitamura YH et al., 1989, “Plant regeneration from callus cultures ofSwertia pseudochinensis” Shoyakugaku Zasshi43:256-258.
Kitamura YH et al., 1988, “Production of swertiamarin in cultured tissues ofSwertia pseudochinensis” Chem. Pharm. Bull. 36:1575-1576.
Kirtikar KM, Basu BD, 1985, Indian Medicinal Plants, Bishen Singh Mahendrapal Singh, Dehradun.
Chopra RN, 1982, Indigenous Drugs of India. Academic Publishers, Calcutta.
Murashige T et al., 1962, “A revised medium for rapid growth and bioassays with tobacco tissue cultures”Plant Physiol. 15:473-493.
Dalall SR, Shah RG, 1956,Chemistry and Industry664.
Rorte F, Schicke HG, 1956,Chem. Ber. 89:2404.
Korte F, 1955,Chem. Ber. 88:704.
Kirtikar KM, Basu BD, 1980, Indian Medicinal Plants, Bishen Singh Mahendrapal Singh, Dehradun, table of contents.
Kirtikar KM, Basu BD, 1980, Indian Medicinal Plants, Bishen Singh Mahendrapal Singh, Dehradun, pp. 1651-1671.
Ahuja Ashok
Kaul Brij Lal
Kaul Mahrak Krishen
Koul Sushma
Qazi Ghulam Nabi
Baker & Botts LLP
Campell Bruce R.
Council of Scientific & Industrial Research Department of B
McCormick S
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