Methods and compositions for purification of nucleic acid...

Details Methods and compositions for purification of nucleic acid... Methods and compositions for purification of nucleic acid...

2011-01-04

2011-01-04

Wallenhorst, Maureen M (Department: 1797)

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

C436S063000, C436S174000, C436S175000, C436S177000, C436S178000, C435S006120, C435S029000, C435S270000, C536S025400

Reexamination Certificate

active

07863050

ABSTRACT:
Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 μm.

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