Methods and compositions for production of lipo-chito...

Plant protecting and regulating compositions – Plant growth regulating compositions – Micro-organisms or from micro-organisms

Reexamination Certificate

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C435S252200, C424S093400

Reexamination Certificate

active

10146034

ABSTRACT:
Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells areBradyrhizobium japonicumcells having the identifying characteristics ofB. japonicumstrain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

REFERENCES:
patent: 5549718 (1996-08-01), Lerouge et al.
patent: 5922316 (1999-07-01), Smith et al.
patent: 5935809 (1999-08-01), Ryan et al.
patent: 2243669 (2000-01-01), None
patent: 2285727 (2001-04-01), None
patent: 00/04778 (2000-02-01), None
patent: 01/26465 (2001-04-01), None
Rosas, Susana et al., “Jasmonic acid stimulates the expression ofnodgenes inRhizobium”, Plant Molecular Biology (1998), 1161-1168.
Ortel, B., “Jasmonate-induced gene expression of barley (Hordeum vulgare) leaves—the link between jasmonate and abscisic acid”, Plant Growth Regulation (1999), 29:113-122.
Mabood, Fazli, Jasmonates induce the expressoin ofnodgenes inBradyrhizobium japonicum, May 17, 2001.
Mabood, Fazli, Linoleic and linolenic acid induce the expression ofnodgenes inBradyrhizobium japonicumUSDA 3, May 17, 2001.

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