Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-07-27
1996-09-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
530412, 530418, C07K 114, C07K 130, C12P 2102
Patent
active
055523021
ABSTRACT:
The in vitro cloning of a gene for human placental ribonuclease inhibitor may be obtained by recombinant DNA technology. The method described obtains the gene encoding human placental ribonuclease inhibitor, the expression of that product in a host cell, and the refolding and purifying of the product. A vector which includes an encoded DNA gene sequence coding for human placental ribonuclease inhibitor is also described, as well as a host that is compatible with and contains the vector. The gene sequence of the human placental ribonuclease inhibitor is also presented.
REFERENCES:
patent: 4673641 (1987-06-01), George et al.
patent: 4734362 (1988-03-01), Hung et al.
patent: 4923967 (1990-05-01), Bobbitt et al.
patent: 4966964 (1990-10-01), Shapiro et al.
patent: 5019556 (1991-05-01), Shapiro et al.
patent: 5082775 (1992-01-01), McCaman et al.
patent: 5220013 (1993-06-01), Ponte et al.
Marston et al., Meth. Enzymol. 182:264-276 (1990).
Saitoh et al., J. Biochem. 101:1281-1288 (1987).
Crawford et al., Gene 85:525-531 (1989).
Marston et al., Biochem. J. 240:1-12 (1986).
Kane et al., Trends Biotechnol. 6:95-101 (1988).
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982, pp. 89-92.
Marston, "The Purification of Eukaryotic Polypeptides Expresses in Eschericia coli," in DNA Cloning: A Practical Approach, D. M Glover, ed., IRL Press, Oxford, England, vol. III, Chap. 4, pp. 59-88 (1987).
Lee et al. "Correction," Biochemistry 28, 7138 (1989).
Lee, et al., Biochemistry, 1989, vol. 28, pp. 225-230, ibid pp. 219-224.
Lee, F. S. and Valle, B. L., Biochemical and Biophysical Research Communications, 1989, vol. 160(1), pp. 115-120.
Young, Richard A. and Ronald W. Davis, "Efficient Isolation of Genes by Using Antibody Probes," Proc. Natl. Acad. Sci. U.S.A., 80:1194-1198 (1983).
Hoess, Adolf, et al., "Recovery of Soluble, Biologically Active Recombinant Proteins from Total Bacterial Lysates Using Ion Exchange Resin," Biotechnology, 6:1214-1217 (1988).
Blackburn, Peter, et al., "Ribonuclease Inhibitor from Human Placenta," The Journal of Biological Chemistry, 252/16:5904-5910 (1977).
Shapiro, Robert and Bert L. Vallee, "Human Placental Ribonuclease Inhibitor Abolishes both Angiogenic and Ribonucleolytic Activities of Angiogenin, " Proc. Natl. Acad. Sci. U.S.A., 84:2238-2241 (1987).
Townbin, Harry, et al., "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications," Proc. Natl. Acad. Sci. U.S.A., 76/9:4350-4354 (1979).
Rosenberg, Alan H., et al., "VEctors for Selective Expression of Cloned DNAs by T7 RNA Polymerase," Gene, 56:125-135 (1987).
Studier, F. William and Barbara A. Moffatt, "Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-level Expression of Cloned Genes," J. Mol. Biol., 189:113-130 (1986).
Laemmli, U. K., "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," Nature, 227:680-685 (1970).
Stark, Michael J. R., "Multicopy Expression Vectors Carrying the lac Repressor Gene for Regulated High-Level Expression of Genes in Escherichia coli," Gene, 51:255-267 (1987).
Inouye, Massayori, "Experimental Manipulation of Gene Expression," Ed., Academic Press, pp. 15-32 (1983).
Movva, N. Rao, et al., "Gene Sructure of the OmpA Protein, a Major Surface Protein of Escherichia coli Required for Cell-Cell Interaction," J. Mol. Biol., 143:329-334 (1980).
Blackburn, Peter, "Ribonuclease Inhibitor from Human Placenta: Rapid Purification and Assay," The Journal of Biological Chemistry, 254/24:12484-12487 (1979).
Blackburn, Peter and Stanford Moore, "The Enzymes, vol. XV, Nucleic Acids, Part B," Ed., Academic Press, pp. 416-424 (1982).
Lee, Frank S., et al., "Primary Structure of Human Placental Ribonuclease Inhibitor," Biochemistry, 27:8545-8553 (1988).
Schneider, Rainer, et al., "The Primary Structure of Human Ribonuclease/ Angiogenin Inhibitor (RAI) Discloses a Novel Highly Dviersified Protein Super-family with a Common Repetitive Module," EMBO Journal, 7/13:4151-4156 (1988).
Erase-a-Base.TM. System Technical Manual (Promega) (1987).
ProtoBlot.RTM. Western Blot AP System TEchnical Manual (Promega) (1987).
ProtoBlot.RTM. Immunoscreening System Technical Manual (Promega) (1987).
Riboprobe.RTM. Gemini System: pGEM-5Zf(+) and pGEM-7Zf(+) Vectors Product Description (Promega) (1988).
LambdaSorb.TM. Phage Adsorbent Product Description (Promega) (1988).
Erase-a-Base.RTM. System Product Description (Promega) (1985).
RNasin.RTM. Ribonuclease Inhibitor (Promega) (1988).
Lewis Martin K.
Shultz John W.
Grimes Eric
Promega Corporation
Wax Robert A.
LandOfFree
Methods and compositions for production of human recombinant pla does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for production of human recombinant pla, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for production of human recombinant pla will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1949218