Methods and compositions for producing recombinant proteins...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S348000, C435S252300, C435S254200

Reexamination Certificate

active

07989181

ABSTRACT:
The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.

REFERENCES:
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patent: 6989265 (2006-01-01), Blattner et al.
patent: WO 00/44926 (2000-08-01), None
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Gourse et al (Proc. Natl. Acad. Sci. USA vol. 82, pp. 1069-1073.
Condon et al 1993. Depletion of functional ribosomal RNA operons inEscherichia colicauses increased expression of the remaining intact copies EMBO J. 12:4305-4315.
Condon, C., C. Squires, and C. L. Squires. 1995. Control of rRNA transcription inEscherichia coli.Microbiol. Rev. 59:623-645.
Brosius, J., et al., “Consruction and Fine Mapping of Recombinant Plasmids Containing the RRNB Ribosomal RNA Operon ofE. coli,” Plasmid, vol. 6, No. 1, pp. 112-118 (1981) XP009098966.
Kiesewetter, S., et al., “Sequences of Three Minor tRNAsARG fromE. coli,” Nucleic Acids Research, vol. 15, No. 7 pp. 3184 (1987) XP002477292.
Roberts, J.W., et al., “Molecular Mechanism for Missense Suppression inE. coli,” Nature, vol. 25, No. 465, pp. 412-414(1974) XP009099376.
International Search Report of PCT/US2007/067232 dated Oct. 29, 2008 .

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