Methods and compositions for producing metabolic products for al

Details Methods and compositions for producing metabolic products for al Methods and compositions for producing metabolic products for al

1991-07-12

1993-12-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435 691, 435 701, 435161, 4351723, 4353201, 435946, 4352402, 536 232, 935 14, 935 23, 935 35, 935 67, 800205, 800200, 800DIG7, C12N 514, C12N 1582, A01H 1300, C12P 706

Patent

active

052701751

ABSTRACT:
Soluble metabolic products, such as ethanol, are produced by growing modified algal cells in a growth medium and recovering the products from the growth medium. The algal cells are modified to overproduce the metabolic product by providing for overexpression of at least one enzyme in the metabolic pathway for the product. For the production of ethanol, the alcohol dehydrogenase gene, the pyruvate decarboxylase gene, or both, are overexpressed, typically under the control of a heterologous promoter. The algal cells may be modified by transformation with a DNA construct including coding sequence(s) of the enzyme(s) under the control of a heterologous promoter.

REFERENCES:
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Bennetzen et al. "The Primary Structure of the Saccharomyces . . . " J. Biol. Chem 257:3018-3025 (Mar. 1982).
Kellermann, E. et al. "Analysis of the Primary Structure and . . . " Nuac Acids Res. 14:8963-8977 (Nov. 1986).
Stokes, H. W. et al. "Recombinant genetic approaches for efficient . . . " Energy Research Abstracts 11(6) Abstract 11145 (Mar. 1986).
Bold, H. C. et al. (eds) "Introduction to the Algae" Prentice-Hall Inc. Englewood Cliffs. N.J. pp. 191-204 (1985).

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