Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Reexamination Certificate
2007-07-31
2007-07-31
Weber, Jon (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
Reexamination Certificate
active
10462091
ABSTRACT:
The present invention concerns the methods and compositions for preparing a tissue section or biological sample, particularly to preserve RNA in the section or sample, by not exposing or contacting the sample or section to a solution that is composed of mostly water. Tissue sections can be fixed, stained, and dehydrated for subsequent manipulation, including laser capture microdissection (LCM) for further analysis using methods and/or compositions of the invention.
REFERENCES:
patent: 5614376 (1997-03-01), Copley et al.
patent: 6316234 (2001-11-01), Bova
patent: 6348325 (2002-02-01), Zahniser et al.
patent: 2002/0006625 (2002-01-01), Chu
patent: 2002/0013222 (2002-01-01), Rei et al.
patent: 2003/0087333 (2003-05-01), Hirai et al.
patent: 2409167 (1975-08-01), None
Tanji, N. et al. 2001. Effect of Tissue Processing on the Ability to Recover Nucleic Acid fronm Specific Renal Tissue Compartments by Laser Capture Microdissection. Experimental Nephrology, vol. 9, pp. 229-234.
“HistoGene™ LCM frozen section staining kit,” Arcturus Catalog #KIT0401, Version A, Arcturus Systems for Microgenomics, 2001 copyright date.
“Optimized protocol for preparing and staining LCM samples from frozen tissue and extraction of high-quality RNA,” Arcturus Application Note #1, found at the Arcturus website arctur.com/images/pdf/HistoGene—Application—Note.pdf, Aug. 2001.
“RNAqueous™ -Micro Instructional manual,” Version 0303, Cat #1927, Ambion, Inc., 2003.
“Staining protocol for LCM samples,” Ambion TechNotes found at http://www.ambion.com/techlib/misc/LCM—staining.html, May 6, 2003.
“Working with laser capture microdissection samples,” Ambion TechNotes, vol. 9, No. 6, Nov. 2002, found at http://www.ambion.com/techlib/tn/96/9616.html, May 6, 2003.
Fend et al., “Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis,”Am. J. Pahtol., 154:61-66, 1999.
Fend et al., “Laser capture microdissection: methodical aspects and applications with emphasis on immuno-laser capture microdissection,”Pathobiology, 68:209-214, 2000.
Fink et al., “Immunostaining and laser-assisted cel picking for mRNA analysis,”Lab. Invest.,80:327-333, 2000.
Fink et al., “Immunostaining for cell picking and real-time mRNA quantitation,”Am. J. Pathol.,157:1459-1466, 2000.
Goldworthy et al., “Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue,”Molecular Carcinogenesis25:86-91, 1999.
Kazumori et al., “Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastic fundic glands using laser capture microdissection,”FEBS Letters489:208-214, 2001.
Kohda et al., “Analysis of segmental renal gene expression by laser capture microdissection,”Kidney International, 57:321-331, 2000.
Lindeman et al., “Gene transcript quantitation by real-time RT-PCR in cells selected by immunohistochemistry-laser capture microdissection,”Diagn. Mol. Pathol.,11:187-192, 2002.
Luzzi et al., “Expression profiling of ductal carcinomal in situ by laser capture microdissection and high-density oligonucleotide arrays,”Am. J. Pathol., 158:2005-2010, 2001.
Murakami et al., “IF-LCM: laser capture microdissection of immunofluorescently defined cells for mRNA analysis rapid communication,”Kidney International, 58:1346-1353, 2000.
Tanji et al., “Effect of tissue processing on the ability to recover nucleic acid from specific renal tissue compartments by laser capture microdissection,”Exp. Nephrol., 9:229-234, 2001.
Trogan et al., “Laser capture microdissection analysis of gene ecpression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice,”Proc. Natl. Acad. Sci, USA,99:2234-2239, 2002.
Vincent et al., “Analysis of neuronal gene expression with laser capture microdissection ,”J. Neurosci. Res.69:578-586, 2002.
Wong et al., “Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection,”Proc. Natl. Acad. Sci., USA, 97:12601-12606, 2000.
Craven et al., “Laser capture microdissection and two-dimmenrsional polyacrylamide gel electrophresis: evaluation of tissue preparation and sample limitations,”Journal of Pathology, 160(3):815-822, 2002.
Goldrick Marianna
Gonzales Juanita C.
Ambion Inc.
Fulbright & Jaworski LLP
Srivastava Kailash C.
Weber Jon
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