Methods and compositions for peptide and protein labeling

Details Methods and compositions for peptide and protein labeling Methods and compositions for peptide and protein labeling

2007-02-06

2007-02-06

Wessendorf, T. D. (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007600, C435S007500, C435S007100, C435S021000, C530S350000

Reexamination Certificate

active

10754911

ABSTRACT:
The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ biotin ligase mutants and biotin analogs recognized by such mutants.

REFERENCES:
patent: 5168057 (1992-12-01), Oh et al.
patent: 5723584 (1998-03-01), Schatz
patent: 5874239 (1999-02-01), Schatz
patent: 5932433 (1999-08-01), Schatz
patent: 5952185 (1999-09-01), Huber et al.
patent: 6265552 (2001-07-01), Schatz
Rhee et al, Protein Science, 13, 3043-50, (2004).
Kwon et al, Protein Science, 2000, 9, 1530-39.
Kwon et al, Protein Science, 2002, 11, 558-570.
Kwon et al, Jrnl. Mol. Biol. 200, 304, 821-833.
Chen et al., “Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase,”Nature Methods, Feb. 2005, pp. 99-104, vol. 2, No. 2.
Beckett et al., “A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation”,Protein Sci., 8(4): 921-929, 1999. Abstract Only.
Chapman-Smith et al., “Molecular biology of biotin attachment to proteins”,J. Nutr., 129: 477S-484S, 1999.
Cornish et al., “Site-specific protein modification using a ketone handle”,J. Am. Chem. Soc., 118: 8150-8151, 1996.
De Boer et al., “Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice”,PNAS, 100(13): 7480-7485, 2003.
Gaietta et al., “Multicolor and electron microscopic imaging of connexin trafficking”,Science, 296: 503-507, 2002.
GENBANK Submission; NIH/NCBI; Accession No. M10123; Apr. 26, 1993 (last submission).
Giriat et al., “Protein semi-synthesis in living cells”,J. Am. Chem. Soc., 125: 7180-7181, 2003.
Griffin et al., “Specific covalent labeling of recombinant protein molecules inside live cells”,Science, 281: 269-272, 1998.
Heinis et al., “Selection of catalytically active biotin ligase and trypsin mutants by phage display”,Protein Engineering, 14(12): 1043-1052, 2001.
Irie et al., “A digital fluorescent molecular photoswitch”,Nature, 420: 759-760, 2002.
Keppler et al., “A general method for the covalent labeling of fusion proteins with small moleculesin vivo”, Nature Biotechnology, 21: 86-89, 2003.
Kiick et al., “Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation”,PNAS, 99(1): 19-24, 2002.
Link et al., “Cell surface labeling ofEscherichia colivia copper(I)-catalyzed [3+2] cycloaddition”,J. Am. Chem. Soc., 125: 11164-11165, 2003.
Looger et al., “Computational design of receptor and sensor proteins with novel functions”,Nature, 423: 185-190, 2003.
Mahal et al., “Engineering chemical reactivity on cell surfaces through oligosaccharide biosynthesis”,Science, 276: 1125-1128, 1997.
Quellhorst et al., “Modification of Rab5 with a photoactivatable analog of geranylgeranyl diphosphate”,J. Biol. Chem., 276(44): 40727-40733, 2001.
Sato et al., “Site-specific modification of interleukin-2 by the combined use of genetic engineering techniques and transglutaminase”,Biochemistry, 35: 13072-13080, 1996.
Saxon et al., “Cell surface engineering by a modified Staudinger reaction”,Science, 287: 2007-2010, 2000.
Schatz et al., “Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation inEscherichia coli”, Biotechnology, 11(10): 1138-1143, 1993. Abstract Only.
Van Delden et al., “A chiroptical molecular switch with distinct chiral and photochromic entities and its application in optical switching of a cholesteric liquid crystal”,Chem. Eur. J., 10: 61-70, 2004.
Van Delden et al., “Photochemical and thermal isomerization processes of a chiral auxiliary based donor—acceptor substituted chiroptical molecular switch: convergent synthesis, improved resolution and switching properties”,Chem. Eur. J., 9: 2845-2853, 2003.
Wang et al., “Bioconjugation by copper (I)-catalyzed azide-alkyne [3+2] cycloaddition”,J. Am. Chem. Soc., 125: 3192-3193, 2003.
Weaver et al., “Corepressor-induced organization and assembly of the biotin repressor: A model for allosteric activation of a transcriptional regulator”,PNAS, 98(11): 6045-6050, 2001.
Wilson et al., “Escherichia colibiotin holoenzyme synthetase /biorepressor crystal structure delineates the biotin- and DNA-binding domains”,Proc. Natl. Acad. Sci. USA, 89: 9257-9261, 1992.
Yeo et al., “Cell-permeable small molecule probes for site-specific labeling of proteins”,Chem. Commun., 2870-2871, 2003.
Zhang et al., “A water-soluble azobenzene cross-linker for photocontrol of peptide conformation”,Bioconjugate Chemistry, 14: 824-829, 2003.
Zhang et al., “A new strategy for the site-specific modification of proteinsin vivo”, Biochemistry, 42: 6735-6746, 2003.

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