Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters plant part growth
Reexamination Certificate
2007-05-11
2011-12-13
Fox, David T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters plant part growth
C800S286000, C800S287000, C800S293000, C800S294000, C800S300000, C800S312000, C435S193000, C435S194000, C435S419000
Reexamination Certificate
active
08076536
ABSTRACT:
The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
REFERENCES:
patent: 5429939 (1995-07-01), Misawa et al.
patent: 5545816 (1996-08-01), Ausich et al.
patent: 5618988 (1997-04-01), Huptmann et al.
patent: 5731179 (1998-03-01), Komari et al.
patent: 6307123 (2001-10-01), Kriz et al.
patent: 6323396 (2001-11-01), Dirks et al.
patent: 6429356 (2002-08-01), Shewmaker
patent: 6458594 (2002-10-01), Baszczynski et al.
patent: 6583338 (2003-06-01), McElroy et al.
patent: 2003/0110532 (2003-06-01), Armstrong et al.
patent: 2004/0072323 (2004-04-01), Matsuda et al.
patent: 2004/0237142 (2004-11-01), Gilbertson et al.
patent: 2006/0041956 (2006-02-01), Lassner et al.
patent: 2006/0064772 (2006-03-01), Kriz et al.
patent: 2006/0200878 (2006-09-01), Lufiyya et al.
patent: 1597969 (2005-02-01), None
patent: 1654662 (2005-08-01), None
patent: 0 687 730 (1995-12-01), None
patent: WO 97/14807 (1997-04-01), None
patent: WO 97/41228 (1997-11-01), None
patent: WO 03/003816 (2003-01-01), None
patent: WO 2004/092390 (2004-10-01), None
patent: WO 2005/079389 (2005-09-01), None
Jianyue et al. Chinese Science Bulletin 50(1): 39-44 (Jan. 2005).
de Pater et al. The Plant Journal 2(6): 837-844 (1992).
Russell et al. Transgenic Research 6: 157-168 (1997).
Kalla et al. Plant Journal 6: 849-860 (1994).
Straub et al. Plant Molecular Biology 26: 617-630 (1994).
Hill et al. Journal of Biological Chemistry 271: 3366-3374 (1996).
Breitler et al., “A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (Oryza sativaL.),”Transgenic Res., 13:271-287, 2004.
Caimi et al., “Fructan accumulation and sucrose metabolism in transgenic maize endosperm expressing a bacillus amyloliquefaciens sacB gene,”Plant Physiol., 110:355-363, 1996.
Carmi et al., “Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary,”Planta, 217:726-735, 2003.
Collens et al., “Development of auxotrophic agrobacterium tumefaciens for gene transfer in plant tissue culture,”Biotechnology Progress, 20:890-896, 2004.
Dale et al., “Gene transfer and subsequent removal of the selection gene from the host genome,”Proc. Natl. Acad. Sci. USA, 88:10558-10562, 1991.
De Buck et al., “T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by agrobacterium-mediated transformation,”Molecular Breeding, 6(5):459-468, 2000.
De Buck et al., “The DNA sequences of T-DNA junctions suggest that complex T- DNA loci are formed by a recombination process resembling T-DNA integration,”Plant J., 20:295-304, 1999.
De Vetten et al., “A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop,”Nat. Biotechnol., 21:439-442, 2003.
Ebinuma et al., “Selection of marker-free transgenic plants using the isopentenyl transferase gene,”Proc. Natl. Acad. Sci. USA, 94:2117-2121, 1997.
Fitzgibbon et al., “Cloning of a gene frompseudomonassp. strain PG2982 conferring increased glyphosate resistance,”Appl. Environ. Microbiol., 56(11):3382-3388, 1990.
GenBank Accession AB101655, Jan. 15, 2008.
GenBank Accession AB101656, Feb. 15, 2008.
GenBank Accession AB101657, Feb. 15, 2008.
GenBank Accession AE009418, May 28, 2004.
GenBank Accession AE009432, May 28, 2004.
GenBank Accession AF060518, Oct. 28, 1998.
GenBank Accession AF400123, Oct. 7, 2001.
GenBank Accession AM422760, Jan. 10, 2007.
GenBank Accession D83391, Sep. 21, 2002.
GenBank Accession L47479, Dec. 29, 1997.
GenBank Accession M15077, Apr. 26, 1993.
GenBank Accession M26194, Apr. 26, 1993.
GenBank Accession M38423, Apr. 11, 2001.
GenBank Accession M87280, Apr. 11, 2001.
GenBank Accession NC—003423, Jan. 25, 2008.
GenBank Accession NM—101474, Apr. 20, 2007.
GenBank Accession NM—119937, Apr. 20, 2007.
GenBank Accession NM—180024, Apr. 20, 2007.
GenBank Accession NM—180913, Apr. 20, 2007.
GenBank Accession NM—202110, Apr. 20, 2007.
GenBank Accession NM—202701, Apr. 20, 2007.
GenBank Accession NM—203251, Apr. 20, 2007.
GenBank Accession U12546, Apr. 6, 1995.
GenBank Accession U13043, Jun. 14, 2002.
GenBank Accession X14202, Jun. 12, 2006.
GenBank Accession X64255, Feb. 10, 1999.
GenBank Accession Y13658, Nov. 14, 2006.
Hajirezaei et al., “Inhibition of potato tuber sprouting: low levels of cytosolic pyrophosphate lead to non-sprouting tubers harvested from transgenic potato plants,”Potato Res., 42:353-372, 1999.
Halpin, “Gene stacking in transgenic plants—the challenge for the 21stcentury plant biotechnology,”Plant Biotechnol. J., 3:141-155, 2005.
Hanson et al., “A simple method to enrich an agrobacterium-transformed population for plants containing only T-DNA sequences,”Plant J., 19:727-734, 1999.
Hare et al., “Excision of selectable marker genes from transgenic plants,”Nature Biotechnol., 20:575-580, 2002.
He et al., “A T42M substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate,”Biosici Biotechnol Biochem., 67(6):1405-1409, 2003.
Horsch et al., “Rapid assay of foreign gene expression in leaf discs transformed by agrobacterium tumefaciens: role of T-DNA borders in the transfer process,”PNAS USA, 83:4428-4432, 1986.
Jofuku et al., “Control of seed mass and seed yield by the floral homeotic gene APETALA2 ,”Proc. Nat. Acad. Sci. USA, 102:3117-3122, 2005.
Kononov et al., “Integration of T-DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration,”Plant J., 11:945-957, 1997.
Mendoza et al., “Leafy cotyledon 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in arabidopsis leaves,”FEBS Letters, 579:4666-4670, 2005.
Misawa et al., “Elucidation of the erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed inEscherichia coli,” J. Bacteriol., 172:6704-6712, 1990.
Mizukami et al., “Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis,”Proc. Natl. Acad. Sci. USA, 97:942-947, 2000.
Morello et al., “Overexpression of the calcium-dependent protein kinase OsCDPK2 in transgenic rice is expressed by light in leaves and disrupts seed development,”Transgenic Res., 9:453-462, 2000.
Ow et al., “Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants,”Science, 234:856-859, 1986.
Puchta, “Marker-free transgenic plants,”Pl. Cell Tiss. Org. Cult., 74:123-143, 2003.
Radchuk et al., “Repressing the expression of the sucrose nonfermenting-1-related protein kinase gene in pea embryo causes pleiotropic defects of maturation similar to an abscisic acid-insensitive phenotype1,”Plant Physiol., 140:263-278, 2006.
Ramanathan et al., “Transfer of non-T-DNA portions of agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA,”Plant Molecular Biology, 28:1149-1154, 1995.
Rotino et al., “Genetic engineering of parthenocarpic plants,”Nat. Biotechnol.,15:1398-1401, 1997.
Sandmann et al., “New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from erwinia species,”FEMS Microbiol Lett., 69(3):253-257, 1992.
Sato et al., “Production of γ-linolenic acid and stearidonic acid in seeds of marker-free transgen
Chomet Paul S.
Gilbertson Larry
Huang Shihshieh
Johnson Susan
Petersen Michael W.
Fox David T
McBride, Esq. Thomas P.
Monsanto Technology LLC
SNR Denton US LLP
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