Methods and compositions for multiplex amplification of...

Details Methods and compositions for multiplex amplification of... Methods and compositions for multiplex amplification of...

2006-05-30

2006-05-30

Lu, Frank (Department: 1634)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S024300, C536S025300, C435S006120, C435S091100, C435S091200

Reexamination Certificate

active

07053206

ABSTRACT:
A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield of products. The ratios are determined as a function of the length of the amplicon and the length of other amplicons being simultaneously tested. The primers may desirably be for p53 gene sequences.

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patent: 5700637 (1997-12-01), Southern
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patent: WO 96/01909 (1996-01-01), None
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patent: WO 96/39535 (1996-12-01), None
patent: WO 96/41012 (1996-12-01), None
Haff, PCR Methods and Applications, vol. 3, pp. 332-337 (1994).

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