Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2007-02-08
2010-10-19
Kallis, Russell (Department: 1638)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023400, C536S023200, C536S023100, C435S320100, C435S182000, C800S260000, C800S287000, C800S298000
Reexamination Certificate
active
07816507
ABSTRACT:
The invention provides bifunctional plant biosynthetic enzymes that increase the efficiency by which modification can be made to plant biosynthetic pathways. In certain aspects of the invention, bifunctional isoflavone biosynthetic enzymes are provided. The invention therefore allows the modification of plants for isoflavone content. The inventors have demonstrated increased isoflavone biosynthesis can be obtained even in non-legume plants.
REFERENCES:
patent: 5545818 (1996-08-01), McBride et al.
patent: 2004/0093632 (2004-05-01), Dixon et al.
patent: 2006/0123508 (2006-06-01), Dixon et al.
patent: WO 00/04175 (2000-01-01), None
patent: WO 00/44909 (2000-08-01), None
patent: WO 02/066625 (2002-08-01), None
patent: WO 03/031622 (2003-04-01), None
patent: WO 03/040306 (2003-05-01), None
patent: WO 03/093464 (2003-11-01), None
patent: WO 2004/024079 (2004-03-01), None
patent: WO 2004/090136 (2004-10-01), None
Akashi T. et al. Plant Physiology, Mar. 25, 2005; vol. 137, pp. 882-891.
Lapcik, O. Phytochemistry 2007, vol. 68, pp. 2909-2916.
Batard et al., “Molecular cloning and functional expression in yeast of CYP76B1, a xenobiotic-inducible 7-ethoxycoumarin O-de-ethylase from helianthus tuberosus,”The Plant Journal, 14(1):111-120, 1998.
Didierjean et al., “Engineering herbicide metabolism in tobacco and arabidopsis with CYP76B1, a cytochrome P450 enzyme from jerusalem artichoke,”Plant Physiology, 130:179-189, 2002.
Liu et al., “Metabolic engineering of isoflavonoid biosynthesis in arabidopsis thaliana,”Plant Biology, Abstracts of the Annual Meeting of the American Society of Plant Physiologists, Abst. #269, p. 1, 2000.
Muir et al., “Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols,”Nature Biotechnology, 19:470-474, 2001.
Shiota et al., “Expression of human cytochromes P450 1A1 and P450 1A2 as fused enzymes with yeast NADPH-cytochrome P450 oxidoreductase in transgenic tobacco plants,”Biosci. Biotechnol. Biochem., 64(10):2025-2033, 2000.
Shiota et al., “Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A 1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase,”Plant Physiol., 106:17-23, 1994.
Winkel-Shirley, “Evidence for enzyme complexes in the phenylpropanoid and flavonoid pathways,”Physiol. Plant, 107:142-149, 1999.
Yilmaz et al., “Enhanced stress tolerance inEscherichia coliand nicotiana tabacum expressing a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein,”Biotechnol. Prog., 18:1176-1182, 2002.
Akashi et al., “Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis,”Plant Physiol., 137:882-891, 2005.
Arai et al., “Design of the linkers which effectively separate domains of a bifunctional fusion protein,”Protein Eng., 14:529-532, 2001.
Barnes, “Soy isoflavones—phytoestrogens and what else?,”J. Nutr., 134:1225S-1228S, 2004.
Beaujean et al., “Engineering direct fructose production in processed potato tubers by expressing a bifunctional alpha-amylase/glucose isomerase gene complex,”Biotechnol. Bioeng., 70:9-16, 2000.
Bülow, “Preparation of artificial bifunctional enzymes by gene fusion,”Biochem. Soc. Symp., 57:123-133, 1990.
Cornwell et al., “Dietary phytoestrogens and health,”Phytochemistry, 65:995-1016, 2004.
Deavours and Dixon, “Metabolic engineering of isoflavonoid biosynthesis in alfalfa,”Plant Physiol., 138:2245-2259, 2005.
DellaPenna, “Nutritional Genomics: Manipulating Plant Micronutrients to Improve Human Health,”Science, 285:375-379, 1999.
Dixon and Ferreira, “Genistein,”Phytochemistry, 60:205-211, 2002.
Dixon, “Engineering of plant natural product pathways,”Curr. Opin. Plant Biol., 8:329-336, 2005.
Dixon, “Natural products and plant disease resistance,”Nature, 411:843-847, 2001.
Dixon, “Phytoestrogens,”Annu. Rev. Plant Biol., 55:225-261, 2004.
Galili and Hofgen, “Metabolic engineering of amino acids and storage proteins in plants,”Metab. Eng., 4:3-11, 2002.
Gowri et al., “Stress Responses in Alfalfa (Medicago sativaL.): X. Molecular Cloning and Expression of S-Adenosyl-l-Methionine:Caffeic Acid 3-O-Methyltransferase, a Key Enzyme of Lignin Biosynthesis,”Plant Physiol., 97:7-14, 1991.
Hey et al., “Artificial, non-antibody binding proteins for pharmaceutical and industrial applications,”Trends Biotech., 23(10):514-522, 2005.
Hoffmann et al., “Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism,”J. Biol. Chem., 278:95-103, 2003.
James and Viola, “Production and Characterization of Bifunctional Enzymes. Substrate Channeling in the Aspartate Pathway,”Biochemistry, 41:3726-3731, 2002.
Jang et al., “Expression of a bifunctional fusion of theEscherichia coligenes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth,”Plant Physiol., 131:516-524, 2003.
Jez et al., “Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase,”Nat. Struct. Biol., 7:786-791, 2000.
Keung and Vallee, “Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase,”Proc. Natl Acad. Sci. USA, 90:1247-1251, 1993.
Kim et al., “Construction and evaluation of a novel bifunctional N-carbamylase-D-hydantoinase fusion enzyme,”Appl. Environ. Microbiol., 66:2133-2138, 2000.
Kourtz et al., “A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis,”Plant Biotech J., 3:435-447, 2005.
La Camera et al., “Metabolic reprogramming in plant innate immunity: the contributions of phenylpropanoid and oxylipin pathways,”Immunol. Rev., 198:267-284, 2004.
Li et al., “A novel bifunctional fusion enzyme catalyzing ethylene synthesis via 1-aminocyclopropane1-carboxylic acid,”J. Biol. Chem., 271:25738-25741, 1996.
Liu et al., “Bottlenecks for metabolic engineering of isoflavone glycoconjugates in Arabidopsis,”Proc. Natl. Acad Sci. USA, 99:14578-14583, 2002.
Liu et al., “Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes fromMedicago truncatula,” Plant J., 36:471-484, 2003.
Netzer and Hartl, “Recombination of protein domains facilitated by co-translational folding in eukaryotes,”Nature, 388:343-349, 1997.
Nixon et al., “Hybrid enzymes: manipulating enzyme design,”Trends Biotechnol., 16:258-264, 1998.
Paiva et al., “Stress responses in alfalfa (Medicago sativaL.) 11. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis,”Plant Mol. Biol., 17:653-667, 1991.
Reddy et al., “Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativaL.),”Proc. Natl. Acad. Sci. USA, 102:16573-16578, 2005.
Sawada et al., “Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase,”Plant J., 31:555-564, 2002.
Schoch et al., “CYP98A3 fromArabidopsis thalianais a 3′-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway,”J. Biol. Chem., 276:36566-36574, 2001.
Seo et al., “Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase ofEscherichia coli,” Appl. Environ. Microbio., 66:2484-2490, 2000.
Shimada et al., “A cluster of genes encodes the two types o
Dixon Richard A.
Tian Li
Kallis Russell
Rhines, Esq. Steven P.
Sonnenschein Nath & Rosenthal LLP
The Samuel Roberts Noble Foundation
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