Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2007-10-09
2007-10-09
Baum, Stuart F. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S278000, C800S290000, C800S287000, C800S298000, C435S419000, C426S054000
Reexamination Certificate
active
10253971
ABSTRACT:
In a method for altering a nutritional profile of a plant, a nucleic acid sequence is selected for its ability to encode a protein capable of modifying the utilization of a substrate in the sugar alcohol secondary metabolic pathway of a plant. The protein is non-native to the sugar alcohol secondary metabolic pathway. Plant cells are transformed with an expression cassette containing the nucleic acid sequence. Genetically altered plants are recovered from the plant cells, the plants having an altered nutritional profile relative to wild-type plants. Genetically modified plants having improved nutritional profiles, and obtainable by the above method, are also provided.
REFERENCES:
patent: 5563324 (1996-10-01), Tarczynski et al.
patent: 5589615 (1996-12-01), De Clercq et al.
patent: 5656496 (1997-08-01), Quail et al.
patent: 6399861 (2002-06-01), Anderson et al.
patent: 2006/0053510 (2006-03-01), Alberte et al.
patent: 818138 (1996-03-01), None
patent: 8266179 (1996-10-01), None
patent: WO9114782 (1991-10-01), None
patent: WO9219731 (1992-11-01), None
patent: WO9527068 (1995-10-01), None
patent: WO9639859 (1996-12-01), None
patent: WO9723599 (1997-07-01), None
Loewus et al (2000, Plant Science 150:1-19).
Bohnert et al (1995, The Plant Cell 7:1099-1111).
Kohno-Murase et al (1994, Plant Molecular Biology 26:1115-1124).
Chapple, Clint C.S., et al. “AnArabidopsisMutant Defective in the General Phenylpropanoid Pathway”The Plant Cell, 4:1413-1424, Nov. 1992.
Wood, Andrew J., et al., “Betaine Aldehyde Dehydrogenase in Sorghum”Plant Physiol, 110:1301-1308 (1996).
Burnet, Michael, et al. “Assay, Purification, and Partial Characterization of Choline Monooxygenase from Spinach”Plant Physiol, 108:581-588 (1995).
Bouchereau, Alain, et al. “Structural changes in sinapic acid conjugates during seedling development of rape”,Plant Physiol. Biochem., 30(4):467-475.
Vogt, Thomas, et al. “Purification and Characterization of Sinapine Synthase from Seeds ofBrassica napus”, Archives of Biochemistry and Biophysics, 300(2):622-628, Feb. 1, 1993.
Selvaraj, Gopalan, et al. “Glycinebetaine in Oilseed Rape and Flax Leaves: Detection by Liquid Chromatography/Continuous Flow Secondary Ion-Mass Spectrometry”,Phytochemistry, 38(5):1143-1146, 1995.
Chapple, et al. “Secondary Metabolite Profiles of Crucifer Seeds: Biogenesis, Role, and Prospects for Directed Modification”,Biosynthesis and Molecular Regulation of Amino Acids in Plants, BK Singh, HE Flores, JC Shannon, eds, American Society of Plant Physiologists 239:248, Copyright 1992.
Holmström, Kjell-Ove, et al. “Production of theEscherichia colibetaine-aldehyde dehydrogenase, an enzyme required for the synthesis of the osmoprotectant glycine betaine, transgenic plants”,The Plant Journal, 6(5):749-758, (1994).
Chapple, Clint “Genetic Characterization of Secondary Metabolism inArabidopsis” Genetic Engineering of Plant Secondary Metabolism, Plenum Press, New York, 251:274 (1994).
Goh, Y.K., et al. “Effect of Ammoniation of Rapeseed Meal on the Sinapine Content of the Meal”British Poultry Science, 23:121-128 (1982).
Kräling, K., et al. “Genetic Variation of the Content of Sinapoyl Esters in Seeds of Rape,B. napus” Plant Breeding, 106, 254-257 (1991).
Lorenzen, Maike, et al. “Sinapic Acid Ester Metabolism in Wild Type and a Sinapoylglucose-Accumulating Mutant ofArabidopsis” Plant Physiol. 112:1625-1630 (1996).
Fenwick, G. Roger “A Micromethod for the Screening of Individual Seeds and Cotyledons ofBrassica napusandBrassica campestris(Rapeseed) for Low Sinapine Content”J. Sci. Food Agric.30:661-663 (1979).
Ismail, F. “Bitterness and Astringency of Sinapine and Its Components”Journal of Food and Science46:1241-1244 (1981).
Regenbrecht, Josef “Distribution of 1-Sinapolyglucose: Choline Sinapolytransferase Activity in the Brassicaceae”Phytochemistry24:407-410 (1985).
Hayashi, Hidenori, et al. “Transformation ofArabidopsis thalianawith thecodAgene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress”The Plant Journal12(1):133-142 (1997).
Deshnium, Patcharaporn, et al. “Transformation ofSynechococcuswith a gene for choline oxidase enhances tolerance to salt stress”Plant Molecular Biology29:897-907 (1995).
Li, Jiayang, et al. “ArabidopsosFlavonoid Mutants are Hypersensitive to UV-B Irradiation”The Plant Cell5:171-179 (1993).
Elkind, et al. “Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylalanine ammonia-lyase gene”,Proc. Natl. Acad. Sci.87:9057-9061 (1990).
Kutchan, T.M. “Alkaloid Biosynthesis—The basis for metabolic engineering of medicinal plants”,The Plant Cell7:1059-1070.
Varin, L. “Flavonoid sulfation: Phytochemistry, enzymology and molecular biology,” Phenolic Metabolism in Plants 233-254 (1990).
Lefebvre, D. “Expression of mamalian metallothionein suppresses glucosinolate synthesis inBrassica campestris,” Plant Physiol. 93:522-524.
Smart, et al. “Overexpression of D-myo-inositol-3-phosphate synthase leads to elevated levels of inositol inArabidopsis,”Plant Molecular Biology 33:811-820 (1997).
Boudet, et al. Transley review No. 80, “Biochemistry and molecular biology of lignification”,New Phytol.129:203-236 (1995).
Herbers, et al. “Manipulating metabolic partitioning in transgenic plants”,Trends in Biotechnologyvol. 14.
Lindsey, K., “Prospects for the genetic manipulation of complex metabolic pathways”,Manipulating secondary metabolism in culture, R.J. Robins, MJC Rhodes, eds., AFRC Institute of Arable Crops Research, Norwich Laboratory, Norwich, UK, pp. 123-133.
Yeoman, et al. Transley review No. 90, “Manipulating secondary metabolism in cultured plant cells”,New Phytol134:553-569.
Ibrahim, R.K., “Engineering altered glucosinolate biosynthesis By two alternative strategies”,Genetic Engineering of Plant Secondary Metabolism, 125-152.
Van der Geest, et al. “A 68 bp element of the β-phaseolin functions as a seed-specific enhancer”,Pant Molecular Biologyvol. 324:579-588 (1996).
Smith, et al. “Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes”,Naturevol. 334:724-726 (1988).
Datla Raju S. S.
Dong Jin-Zhuo
Georges Fawzy
Hussain Atta A. K.
Keller Wilfred A.
Bacon & Thomas PLLC
Baum Stuart F.
National Research Council of Canada
LandOfFree
Methods and compositions for modifying levels of secondary... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for modifying levels of secondary..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for modifying levels of secondary... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3852665