Methods and compositions for modification of lipid biosynthesis

Details Methods and compositions for modification of lipid biosynthesis Methods and compositions for modification of lipid biosynthesis

2006-10-10

2006-10-10

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S183000, C435S193000, C435S252300, C435S252330, C435S252340, C435S254110, C435S320100, C435S325000, C435S410000, C536S023200

Reexamination Certificate

active

07118896

ABSTRACT:
The invention provides polypeptides having wax ester synthase and acyl-CoA:diacylglycerol acyltransferase activity. Also provided are the nucleic acids encoding such polypeptides, cells and organisms transformed therewith and methods of use thereof. The invention allows the modification of lipid profiles in host cells and organisms. Novel methods for the production of waxy esters are also provided by the invention.

REFERENCES:
patent: 6143538 (2000-11-01), Somerville et al.
patent: 6492509 (2002-12-01), Lardizabal et al.
patent: WO 93/10241 (1993-05-01), None
Barbe et al., “Unique features revealed by the genome sequence of acinetobacter sp. adp1, a versatile and naturally transformation competent bacterium,”Nucleic Acides Research, 32:5766-5779, 2004.
Coleman et al., “Enzymes of triacylglycerol synthesis and their regulation,”Progress in Lipid Research, 43:134-176, 2004.
EMBL Database XP-002336524.
Kalscheuer et al, “In vitro and invivo biosynthesis of wax diesters by an unspecific bifunctional wax ester synthase/acyl-coa:diacylglycerol acyltransferase from acinetobacter calcoaceticus adp1,”Eur. J. Lipid Sci Technol.I, 105:578-584, 2003.
Kalscheuer et al., “A novel bifunctional wax ester synthase/acyl-coa:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in acinetobacter calcoaceticus adp1,”J of Biological Chemistry, 278, 8075-8082, 2003.
Kalscheuer et al., “Synthesis of novel lipids in saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase,”Applied and Environmental Microbiology, 70:7119-7125, 2004.
Lardizabel et al., “Purification of a jojoba embryo wax synthase, cloning of its cdna, and production of high levels of wax in seeds of trangenic arabidopsis,”Plant Physiology, 122:645-655, 2000.
Stoveken et al., “The was ester synthase/acyl coenzyme a:diacylglycerol acyltransferase from acinetobacter sp. strain adp1: characterization of a novel type of acyltransferase,”J Bacteriology, 187:1369-1376, 2005.
Uthoff et al., “Thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme a:diacylglycerol acyltransferase of acinetobacter sp. strain adp1,”Applied and Environmental Microbiology, 71:790-796, 2005.
Alvarez et al., “Accumulation of storage lipids in species of Rhodococcus and Nocardia and effect of inhibitors and polyethylene glycol,”Fett/Lipid, 9:239-246, 1997.
Cases et al., “Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members,”J. Biol. Chem., 276:38870-38876, 2001.
Chen and Farese, “DGAT and triglyceride synthesis: a new target for obesity treatment,”Trends Cardiovasc. Med., 10:188-192, 2000.
Cole et al., “Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence,”Nature, 393:537-544, 1998.
Cole et al., “Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence,”Nature, 396:190-198, 1998.
Dahlqvist et al., “Phosphlipid: diacylglycerol acyltransferase : an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants,”Proc. Natl. Acad. Sci. USA, 97:6487-6492, 2000.
Fixter et al., “Structure, distribution and function of wax esters inAcinetobacter calcoaceticus,” J. Gen. Microbiol., 132:3147-3157, 1986.
GenBank Accession No. AF149919.
Lehner and Kuksis, “Biosynthesis of triacylglycerols,”Prog. Lipid Res., 35:169-201, 1996.
Lehner and Kuksis, “Triacylglycerol synthesis by an sn-1,2(2,3)-diacylglycerol transacylase from rat intestinal microsomes,”J. Biol. Chem., 268:8781-8786, 1993.
Nakagawa et al., “Metabolism of triacylglycerol inMycobacterium smegmatis,” J. Biochem., 80:923-928, 1976.
Olukoshi and Packter, “Importance of stored triacylglycerols in Streptomyces: possible carbon source for antibiotics,”Microbiology, 140:931-943, 1994.
Reiser and Somerville, “Isolation of mutants ofAcinetobacter calcoaceticusdeficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme a reductase,”J. Bacteriol., 179:2969-2975, 1997.
Stachelhaus et al., “Peptide bond formation in nonribosomal peptide biosynthesis,”J. Biol. Chem., 273:22773-22781, 1998.
Steinbüchel, “Polyhydroxyalkanoic acids,”In Biomaterials, Byrom, (Ed.), Chapter 3: 125-213, MacMillan, London, 1991.
Stobart et al., “Triacylglycerols and synthesised and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctoriusL.) seeds,”Planta, 203:58-66, 1997.
Wun et al., “Neutral lipid biosynthesis inMycobacterium smegmatis,” Biochim. Biophys. Acta, 488:454-463, 1977.
Kalscheuer and Steinbuchel, “A novel bifunctional wax ester synthase/acyl-CoA: diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis inAcinetobacter calcoaceticusADP1,”JBC Papers, In press, published as Manuscript M210533200 on Dec. 26, 2002.

Affiliated with

Also associated with

Rating

Methods and compositions for modification of lipid biosynthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for modification of lipid biosynthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for modification of lipid biosynthesis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3644520