Methods and compositions for linking binding domains in...

Details Methods and compositions for linking binding domains in... Methods and compositions for linking binding domains in...

2002-07-17

2010-12-14

Dunston, Jennifer (Department: 1636)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C536S023100, C536S023400, C435S320100, C514S04400A

Reexamination Certificate

active

07851216

ABSTRACT:
We describe a method of producing a modified nucleic acid binding polypeptide, the method comprising the steps of: (a) providing a nucleic acid binding polypeptide comprising a plurality of nucleic acid binding modules; (b) selecting a first binding domain consisting of one or two contiguous nucleic acid binding modules; (c) selecting a second binding domain consisting of one or two contiguous nucleic acid binding modules; and (d) introducing a flexible linker sequence to link the first and second binding domains, the flexible linker sequence comprising five or more amino acid residues. Use of structured linkers, alone or in combination with flexible linkers, is also disclosed.

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