Methods and compositions for interaction trap assays

Details Methods and compositions for interaction trap assays Methods and compositions for interaction trap assays

2008-07-01

2008-07-01

Shibuya, Mark L. (Department: 1639)

Combinatorial chemistry technology: method, library, apparatus

Library, per se

Library containing only organic compounds

C506S016000, C506S018000, C536S023400, C536S024100

Reexamination Certificate

active

10801994

ABSTRACT:
The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.

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