Methods and compositions for inhibition of RNA splicing

Details Methods and compositions for inhibition of RNA splicing Methods and compositions for inhibition of RNA splicing

2005-10-25

2005-10-25

Fredman, Jeffrey (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100, C435S252800, C435S174000, C435S183000, C382S129000, C382S133000, C382S153000, C382S173000, C382S286000, C382S291000, C702S019000, C702S022000, C536S022100

Reexamination Certificate

active

06958215

ABSTRACT:
A method of inhibiting the self-splicing of a Group I intron is disclosed. The method uses an oligonucleotide having a sequence essentially identical to a guide sequence found in the 5′ flanking exon and terminates with a 3′ ribonucleoside. Usually the oligonucleotide has N3′→P5′ phosphoramidate or N3′→P5′ thiophiosphoramidate linkages rather than phosphodiester linkages. A method of inhibiting the growth of organisms having Group I intron, particularly certain pathogenic fungi includingP. carinii, C. albicans, andA. nidulansusing the oglionucleotide is also provided.

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