Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Reexamination Certificate
2005-06-14
2005-06-14
Wang, Andrew (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
C435S431000
Reexamination Certificate
active
06905876
ABSTRACT:
The present disclosure relates to methods and compositions for in vitro cultivation of species ofPolygonatum, e.g.Polygonatum cirrhifoliumRoyle. The disclosure provides culture media comprising MS basal culture media and plant hormones, preferably selected from the group consisting of gibberellic acid (GA3), 6-benzyl-aminopurine (BAP), and naphthalene acetic acid (NAA). The disclosure provides methods of in vitro cultivation ofPolygonatumcomprising contactingPolygonatumseeds with a first medium comprising MS basal culture medium and GA3, upon emergence of a hypocotyl, transferring this primary explant to a second medium comprising MS basal culture medium, BAP, and NAA, and upon emergence of a first foliage leaf, transferring this secondary explant to a third medium comprising MS basal culture medium, BAP, NAA, and gibberellic acid (GA3). The methods and compositions of the disclosure are capable of inducing and/or supporting uniform germination in less than about 90 days, synchronized development of epicotyl, coleoptile, and radicle, termination of epicotyl dormancy and combinations thereof. The present disclosure relates to the novel culture medium compositions, said compositions comprising Murashige and Skoog (MS), a basal culture medium, varied concentrations of plant hormones, and other additives, leading to extraordinarily fast and synchronized in vitro induction of germination and release of epicotyl dormancy inPolygonatum cirrhifoliumRoyle, an endangered medicinal plant species and a method for faster in vitro propagation ofPolygonatum cirrhifoliumRoyle.
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Dhar Avtar Krishan
Dhar Rekha Sapru
Lattoo Surrinder Kumar
Purohit Paresh
Qazi Ghulam Nabi
Baker & Botts L.L.P.
Council of Scientific and Industrial Research
Para Annette H
Wang Andrew
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