Methods and compositions for in planta production of...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule

Reexamination Certificate

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C800S278000

Reexamination Certificate

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08058509

ABSTRACT:
Methods and compositions are provided which allow for the production of an inverted repeat in a plant or plant part, which, when transcribed as an RNA can be used in some examples to decrease expression of a target polynucleotide of interest. The methods and composition provide precursor inverted repeat cassettes that are not capable of producing a hairpin RNA polynucleotide, and plants and plant parts comprising such precursor inverted repeat cassettes. The precursor inverted repeat cassettes are altered in planta by using a recombination system to produce a inverted repeat. Plants, plant parts, and seeds comprising the various components are also provided.

REFERENCES:
patent: 6262341 (2001-07-01), Baszczynski
patent: 6331661 (2001-12-01), Baszczynski
patent: 6933146 (2005-08-01), Helliwell et al.
patent: 7102055 (2006-09-01), Baszczynski
patent: 2004/0132042 (2004-07-01), Frankard et al.
patent: 2006/0143737 (2006-06-01), Yadav
patent: 1 080 208 (2005-03-01), None
patent: WO 2004/048581 (2004-06-01), None
patent: WO 2006/056617 (2006-06-01), None
Marillonnet et al. 2004, PNAS 101:6852-6857.

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