Methods and compositions for identifying osteogenic agents

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 4, 435 29, 4353201, 435325, 435375, 435455, 536 231, 536 241, C12Q 168, C12Q 102, C07H 2104, C12N 1585

Patent

active

060836903

ABSTRACT:
Methods and compositions for identifying osteogenic agents are disclosed, wherein a bone morphogenetic protein promoter is utilized in an assay system to modulate the production of an assayable product of a reporter gene.

REFERENCES:
patent: 5166058 (1992-11-01), Wang et al.
patent: 5266683 (1993-11-01), Oppermann et al.
patent: 5491084 (1996-02-01), Chalfie et al.
Alam et al., "Reporter genes: Application to the study of mammalian gene transcription", Anal. Biochm. 188(2): 245-254, Aug. 1990.
Ghosh-Choudhury, N. et al., "Expression of the BMP 2 Gene during Bone Cell Differentiation," Critical Reviews in Eukaryotic Gene Expression (1994) 4(2&3):345-355.
Harris, S.E. et al., "Retinoid Regulation of Bone Morphogenetic Protein 4 (BMP 4 or DVR 4): Analysis of the Mouse BMP 4 Gene Promoter by Transfection Into Primary Cultures of Fetal Rat Calvariae (FC) osteoblasts," J Cell Biochem Suppl C (17 Part A) (1993) 159.
Harris, S.E. et al., "Development of Osteoblast Cell Lines from Transgenic Mice Containing Bone Morphogenic Protein 2 (BMP2) Promoter-T-Antigen Constructs: Analysis of BMP 2 Retinoic Acid and 1,25(OH).sub.2 Vitamin D Response Regions in the BMP 2, Promoter in the Context of Chromatin Structure," J Cell Biochem Suppl. C (18B) (1994).
Anderson, H.C. et al., "Bone-Inducing Capability of Cultured Human Cells (FL and HELA) Compared to that of Various Types of Injury," Fed. Proceed. (1968) 27:475.
Bellows, C.G. et al., "Mineralized Bone Nodules Formed In Vitro from Enzymatically Released Rat Calvaria Cell Populations," Calcif Tissue Int (1986) 38:143-154.
Chen, D. et al., "Cloning and sequence of bone morphogenetic protein 4 cDNA from fetal rat calvarial cell," Biochimica et Biophysica Acta (1993) 1174:289-292.
Feng, J.Q. et al., "Structure and sequence of mouse bone morphogenetic protein-2 gene (BMP-2): comparison of the structures and promoter regions of BMP-2 and BMP-4 genes," Biochimica et Biophysica Acta (1994) 221-224.
Frost, E. et al., "Mapping Temperature-Sensitive and Host-Range Mutations of Adenovirus Type 5 by marker Rescue," Virology (1978) 91:39-50.
Gorman, C. "DNA Cloning, A Practical Approach" (Gover, D.M., ed) (1988) vol. II, pp. 157-158, IRL Press, Oxford, England.
Graham, F.L. et al., "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA," Virology (1973) 52:456-467.
Hall, J.A. et al., "Acidic Fibroblast Growth Factor Gene 5' Non-Coding Exon and Flanking Region From Hamster DDT1 Cells: Identification of the Promoter Region and Transcriptional Regulation by Testosterone and aFGF Protein," Journal of Cellular Biochemistry (1993) 51:116-127.
Harris, S.E. et al., "Expression of Bone Morphogenetic Protein Messenger RNAs by Normal Rat and Human Prostate and Prostate Cancer Cells," The Prostate (1994) 24:204-211.
Harris, S.E. et al., "Effects of Transforming Growth Factor .beta. on Bone Nodule Formation and Expression of Bone Morphogenetic Protein 2, Osteocalcin, Osteopontin, Alkaline Phosphatase, and Type I Collagen mRNA in Long-Term Cultures of Fetal Rat Calvarial Osteoblasts," Journal of Bone and Mineral Research (1994) 9(6):855-863.
Harris, S.E. et al., "Expression of Bone Morphogenetic Protein Messenger RNA in Prolonged Cultures of Fetal Rat Calvarial Cells," Journal of Bone and Mineral Research (1994) 9(3):389-394.
Kurihara, T. et al., "Murine Bone Morphogenetic Protein-4 Gene: Existence of Multiple Promoters and Exons for the 5'-Untranslated Region," Biochemical and Biophysical Research Communications (1993) 192(3):1049-1056.
Luckow, B., "CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements," Nucleic Acids Res (1987) 15:5490.
Majeska, R.J. et al., "Maintenance of parathyroid hormone response in clonal rat osteosarcoma lines," Exp Cell Res (1978) 111:465-467.
Majeska, R.J. et al., "Parathyroid Hormone-Responsive Clonal Cell Lines from Rat Osteosarcoma," Endocrinology (1980) 107(5):1494-1502.
Potter, H., "Electroporation in Biology: Methods, Applications, and Instrumentation," Analytical Biochemistry (1988) 174:361-373.
Sanger, F. et al., "DNA sequencing with chain-terminating inhibitors," Proc Natl Acad Sci USA (1977) 74(12) 5463-5467.
Stein, G.S. et al., "Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation," Cell Growth and Gene Expression (1990) 4:3111-3123.
Urist, M.R., "Bone: Formation by Autoinduction," Science (1965) 150:893-899.
van den Hoff, M.J.B. et al., "Electroporation in `intracellular` buffer increases cell survival," Nucleic Acids Research (1992) 20(11):2902.
Wozney, J.M. et al., "Novel Regulators of Bone Formation: Molecular Clones and Activities," Science (1988) 242:1528-1534.
Wozney, J.M., "The Bone Morphogenetic Protein Family and Osteogenesis," Molecular Reproduction and Development (1992) 32:160-167.
Wozney, J.M. et al., "Bone Morphogenetic Proteins," In: Physiology and Pharmacology of Bone (edited by Mundy, G.R., Martin, T.J.). Springer-Verlag, Chapter 20, (1993) pp. 725-743.
Feng, J.Q. et al., "The Mouse Bone Morphogenetic Protein-4 Gene,"Journal of Biological chemistry,vol. 270, No. 47, Nov. 24, 1995, pp. 28364-28373.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods and compositions for identifying osteogenic agents does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for identifying osteogenic agents, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for identifying osteogenic agents will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1484906

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.