Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-05-31
2005-05-31
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S287200, C536S023100, C536S024300, C536S024330
Reexamination Certificate
active
06900013
ABSTRACT:
The present invention recognizes that identifying genes expressed during developmental processes, stress responses, and disease states can advance understanding of these biological functions, and can contribute to identifying targets for therapeutic drugs. In addition, the present invention recognizes that rapid and reliable profiling of genetic variations, such as mutations and SNPs, is of increasing importance to diagnostics, prognostics, forensics, heredity determinations, and pharmacogenetics. One aspect of the present invention provides a method of identifying one or more nucleic acid molecules that are expressed under a given set of conditions based on their complementarity to known sequences, or one or more mutations or SNPs in a population of nucleic acid molecules. The method includes: contacting at least one probe nucleic acid molecule with a survey population of nucleic acid molecules under conditions that promote nucleic acid hybridization to generate a probe-survey population mixture of nucleic acid molecules, treating the probe-survey population mixture of nucleic acid molecules with a nucleolytic activity, such that nucleolytic activity-sensitive nucleic acid molecules are digested, and contacting the resulting mixture of nucleolytic activity-protected nucleic acid molecules with a solid support comprising one or more attached nucleic acid molecules to generate attached nucleic acid molecule
ucleolytic activity-protected nucleic acid molecule complexes, and identifying one or more of the attached nucleic acid molecules or one or more of the nucleolytic activity-protected nucleic acid molecules in one or more attached nucleic acid molecule
ucleolytic activity-protected nucleic acid molecule complexes.
REFERENCES:
patent: 3687808 (1972-08-01), Merigan, Jr. et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5242974 (1993-09-01), Holmes
patent: 5359115 (1994-10-01), Campbell et al.
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5420328 (1995-05-01), Campbell
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5436327 (1995-07-01), Southern et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5472672 (1995-12-01), Brennan
patent: 5527681 (1996-06-01), Holmes
patent: 5529756 (1996-06-01), Brennan
patent: 5532128 (1996-07-01), Eggers et al.
patent: 5545531 (1996-08-01), Rava et al.
patent: 5554501 (1996-09-01), Coassin et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5561071 (1996-10-01), Hollenberg et al.
patent: 5571639 (1996-11-01), Hubbell et al.
patent: 5593839 (1997-01-01), Hubbell et al.
patent: 5599695 (1997-02-01), Pease et al.
patent: 5624711 (1997-04-01), Sundberg et al.
patent: 5629156 (1997-05-01), Shah et al.
patent: 5658734 (1997-08-01), Brock et al.
patent: 5681943 (1997-10-01), Letsinger et al.
patent: 5700637 (1997-12-01), Southern
patent: 5770370 (1998-06-01), Kumar
patent: 5773257 (1998-06-01), Nielson et al.
patent: 5888819 (1999-03-01), Goelet et al.
patent: 5952174 (1999-09-01), Nikiforov et al.
patent: 5994076 (1999-11-01), Chenchik et al.
patent: 5994079 (1999-11-01), De La Rosa et al.
patent: 5998136 (1999-12-01), Kamb
patent: 6004744 (1999-12-01), Goelet et al.
patent: 6004752 (1999-12-01), Loewy et al.
patent: 6004755 (1999-12-01), Wang
patent: 6013431 (2000-01-01), Soderlund et al.
patent: 6228580 (2001-04-01), Blumenfeld et al.
patent: 6232066 (2001-05-01), Felder et al.
patent: 6238869 (2001-05-01), Kris et al.
patent: 6268146 (2001-07-01), Shultz et al.
patent: 6448010 (2002-09-01), Zhao
patent: 6458533 (2002-10-01), Felder et al.
patent: 2003/0096232 (2003-05-01), Kris et al.
patent: WO 95/21944 (1995-08-01), None
patent: WO 99/32663 (1999-07-01), None
patent: WO 00/34521 (2000-06-01), None
Tyagi et al., Molecular beacons: probes that fluoresce upon hybridization. Nature Biotechnology 14, 303-308, Mar. 1996.*
Alizah et al., Nature, 403:503-511 (2000).
Arribas et al., Clin. Cancer Res., 5:3454-3459 (1999).
Beaucage and Iyer, Tetrahedron, 48:2223-2311 (1992).
Beaucage and Iyer, Tetrahedron, 49:6123-6194 (1993).
Debouck and Goodfellow, Nature Genetics Supple., 21:48-50 (1999).
Duggan et al., Nature Genetics Supple, 21:10-14 (1999).
Englisch et al., Angewandte Chemie., International Edition, 30:613-629 (1991).
Gerhold et al., Trends Biochem. Sci., 24:168-173 (1999).
Haines and Gillispie, Biotechniques, 12:736-741 (1992).
Lau et al., Anal. Biochem., 209:360-366 (1993).
Martin, Helv. Chim. Acta, 78:486-504 (1995).
Pollack et al., Nature Genetics, 23:41-46 (1999).
Rosenthal and Jones, Nucleic Acids Res., 18:3095-3096 (1990).
Schena et al., Science, 270:467-470 (1995).
Sinha and Cook, Nucleic Acids Res., 16:2659-2669 (1988).
Smith et al., Nucleic Acids Res.,13:2399-2412 (1985).
Strauss and Jacobowitz, Brain Res. Mol. Brain Res., 20:229-239 (1993).
Tanner et al., Clin. Cancer Res., 1:1455-1461 (1995).
Thiesen et al., Tetrahedron Letters, 33:5033-5036 (1992).
Walmsley and Patient, Mol. Biotechnol., 1:265-275 (1994).
Nikiforv et al., Nucleic Acid Research, 22:20:4167-4175 (1994).
Liu, et al., Nature, 403:175-79 (2000).
Surryr, Xenobiotica, 29(8):827-36 (1999) (Abstract).
Cheung and Nelson, Genomics, 47:1-6 (1998).
Pease et al., Proc. Natl. Acad. Sci., 91:5022-5026 (1994).
Tyagi et al., Nature Biotechnol., 14:300-307 (1996).
Cheng Jing
Wang Guoqing
Wang Xiaobo
Wu Lei
Yang WeiPing
AVIVA Biosciences Corporation
David R Preston & Associates APC
Lu Frank
Orr Elizabeth
Whisenant Ethan
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