Methods and compositions for identifying ligands for nuclear...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S320100

Reexamination Certificate

active

07037656

ABSTRACT:
A yeast-based system and methods of use therefore are provided for identifying new molecules which activate nuclear receptors in a ligand-dependent fashion. In a preferred embodiment, a method is provided utilizing ecdysone receptor, USP and GRIP I encoding expression vectors which may be used to advantage for screening new and useful insecticidal compounds, detecting insecticidal residues as well as to regulate expression of a gene of interest in a host in a ligand-dependent manner.

REFERENCES:
patent: WO 97/45737 (1997-12-01), None
Tran, Hiep T., et al., “Reconstruction of Ligand-Dependent Transactivation ofChoristoneura fumiferanaEcdysone Receptor in Yeast”, Molecular Endocrinology 15(7): 1140-1153 (2001).
Tran, Hiep T., et al., “Requirement of co-factors for the ligand-mediated activity of the insect ecdysteriod receptor in yeast”, Journal of Molecular Endocrinology, 27: 191-209 (2001).
Dela Cruz, Fernando, et al., “Drosophila Ecdysone Receptor Functions as a Constitutive Activator in Yeast”, J. Steroid Biochem. Molec. Biol. 62(4):353-359 (1997).
Walfish, Paul G., et al., “Yeast hormone response element assays detect and characterize GRIP1 coactivator-dependent activation of transcription by thyroid and retinoid nuclear receptors”, Proc. Natl. Acad. Sci. USA 94: 3697-3702 (1997).

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