Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2005-11-15
2005-11-15
McElwain, Elizabeth F. (Department: 1638)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C435S243000, C435S440000, C435S471000, C435S469000, C536S023100
Reexamination Certificate
active
06964866
ABSTRACT:
Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such asAgaricus bisporus. The preferred method uses anAgrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.
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Chen Xi
Romaine C. Peter
Helmer Georgia
McElwain Elizabeth F.
McKees, Voorhees & Sease, P.L.C.
The Penn State Research Foundation
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