Methods and compositions for high throughput identification...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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10813639

ABSTRACT:
Methods and compositions for high-throughput identification of protein
ucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein
ucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein
ucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.

REFERENCES:
patent: 5753439 (1998-05-01), Smith et al.
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6355421 (2002-03-01), Coull et al.
patent: 6713262 (2004-03-01), Gellibolian et al.

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