Methods and compositions for high resolution HLA typing

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 221, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 1900, C07H 2104

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active

059087498

ABSTRACT:
Methods and compositions are provided for high resolution HLA typing using microsatellite repeats in the HLA region of the genome. Alleles from these markers are shown to be in tight linkage disequilibrium with HLA-DQ/DR alleles. The methods can be carried out on amplified nucleic acid produced by the polymerase chain reaction using the novel primers of the present invention. The microsatellite marker characterization may be employed in the association of diseases to HLA. The typing methods may be further employed in determining the suitability of a donor for tissue transplantation, including bone marrow transplantation, and in determining an individual's susceptibility to HLA associated diseases, particularly autoimmune diseases.

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