Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-05
1997-05-20
Adams, Donald E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351722, 4353201, 435 7021, 4352523, 5303873, 530867, 536 2353, 536 241, 536 2372, C12N 516, C12N 1509, C12N 1513, C12N 1533
Patent
active
056311583
ABSTRACT:
Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, contitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.
REFERENCES:
patent: 4656134 (1987-04-01), Ringold
patent: 4740761 (1988-04-01), Kaufman
patent: 4956288 (1990-09-01), Barsoum
patent: 5002874 (1991-03-01), Kaufman
patent: 5024939 (1991-06-01), Gorman
patent: 5091513 (1992-02-01), Huston et al.
patent: 5238820 (1993-08-01), Kaufman
Foecking, et al. (1986) "Powerful and Versatile Enhancer-Promoter Unit For Mammalian Expression Vectors," Gene 45:101-105.
Cockett, et al. (1990) "High Level Expression of Tissue Inhibitor Of Metalloproteinases In Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification," Biotechnology 8:662-667.
Cockett, et al. (1991) "The Use of Engineered E1A Genes To Transactivate The hCMV-MIE Promoter In Permanent CHO Cell Lines," Nucleic Acids Res. 19:319-325.
McCartney, et al. (1991) "Biosynthetic Antibody Binding Sites: Development of a Single-Chain Fv Model Based On Antidinitrophenol IgA Myeloma MOPC 315," Journal of Protein Chem. 10:669-683.
Adams, et al. (1993) "Highly Specific in vivo Tumor Targeting by Monovalent and Divalent Forms of 741F8 Anti-c-erbB-2 Single-Chain Fv," Cancer Research 53:4026-4034.
Huston, et al. (1993) "Medical Applications of Single-Chain Antibodies," Intern. Rev. Immunol. 10:195-217.
Grinnell et al., "Transactivated Expression of Fully Gamma-Carboxylated Recombinant Human Protein C, An Antithrombotic Factor" Biotechnology 5:1189-1192 (1987).
Shepard et al. [J. Clin. Immunol. 11(3) 117-127 (1991)].
West et al. [Virology 10:38-47 (1987)].
Wu et al. [Cell 33:77-83 (1983)] Yodoi et al. [J. Immunol. 131(1):303-310 (1983)].
Maurer [Animal Cell Culture: a practical approach, ed. Freshney, chapter 2 pp. 13-31 (1986)].
Yodi et al. JI 131:303-310 (1983).
Rosen et al. (1992), "The BMP Proteins In Bone Formation and Repair," 8 Trends In Genetics 3:97-102.
Wong et al. (1985), "Human GM-CSF: Molecular Cloning of Complementary DNA and Purification of the Natural and Recombinant Proteins," 228 Science 17:810-815.
Datta et al. (1991), "A Purified Adenovirus 289-Amino-Acid E1A Protein Activates RNA Polymerase III Transcription In Vitro and Alters Transcription Factor TFIIIC," 65 J. Virology 10:5297-5304.
Fujita et al. JP 63033386 A2, Chem. Abst.
Dorai Haimanti
Oppermann Herrmann
Adams Donald E.
Creative BioMolecules, Inc.
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