Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-11-27
1998-01-27
Ketter, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 435350, 435353, 435360, C12P 2102, C12N 510, C12N 1564
Patent
active
057121192
ABSTRACT:
Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, contitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.
REFERENCES:
patent: 4656134 (1987-04-01), Ringold
patent: 4740461 (1988-04-01), Kaufman
patent: 4956288 (1990-09-01), Barsoum
patent: 5002874 (1991-03-01), Kaufman
patent: 5024939 (1991-06-01), Gorman
patent: 5238820 (1993-08-01), Kaufman
patent: 5258287 (1993-11-01), Baxter et al.
patent: 5354557 (1994-10-01), Oppermann et al.
patent: 5585237 (1996-12-01), Oppermann et al.
Perides et al. (1994), "Regulation of Neural Cell Adhesion Molecule and L1 by the Transforming Growth Factor-.beta. Superfamily," 269 J. Biol. Chem. 1:765-770.
Ten Dijke et al. (1994), "Identification of Type I Receptors For Ostegenic Protein-1 and Bone Morphogenetic Protein-4," 269 J. Biol. Chem. 25:16985-16988.
Rosen et al. (1992), "The BMP Proteins In Bone Formation and Repair," 8 Trends In Genetics 3:97-102.
Datta et al. (1991), "A Purified Adenovirus 289-Amino-Acid E1 A Protein Activates RNA Polymerase III Transcription In Vitro and Alters Trascription Factor TFIIIC," J. Virology 10:5297-5304.
Cockett, et al. (1991), "The Use of Engineered E1A Genes to Transactivate The hCMV-MIE Promotor In Permanent CHO Cell Lines," Nucleic Acids Res. 19:319-325.
Cockett, et al. (1990) "High Level Expression of Tissue Inhibitor Of Metalloproteinases In Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification," Biotechnology 8:662-667.
Grinnell et al. (1987), "Transactivated Expression of Fully Gamma-Carboxylated Recombinant Human Protein C, An Antithrombotic Factor" Biotechnology 5:1189-1192
West et al. (1987), "Gene Expression in Adeno-Associated Virus Vectors: The Effects of Chimeric mRNA Structure, Helper Virus, and Adenovirus VA.sub.1 RNA," 160 Virology 38-47.
Tibbetts et al. (1986), "Autoregulation of Adenovirus E1A Gene Expression," 57 J. Virology 3:1055-1064.
Foecking, et al. (1986) "Powerful and Versatile Enhancer-Promotor Unit For Mammalian Expression Vectors," Gene 45:101-105.
Wong et al. (1985), "Human GM-CSF: Molecular Cloning of Complementary DNA and Purification of the Natural and Recombinant Proteins," 228 Science 17:810-815.
Dorai Haimanti
Kaplan Paul
Oppermann Hermann
Creative BioMolecules, Inc.
Ketter James
LandOfFree
Methods and compositions for high protein production form recomb does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for high protein production form recomb, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for high protein production form recomb will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-340984